TY - JOUR
T1 - Surface functionalisation-dependent adverse effects of metal nanoparticles and nanoplastics in zebrafish embryos
AU - Hansjosten, Iris
AU - Takamiya, Masanari
AU - Rapp, Juliane
AU - Reiner, Luisa
AU - Fritsch-Decker, Susanne
AU - Mattern, Dorit
AU - Andraschko, Silvia
AU - Anders, Chantal
AU - Pace, Giuseppina
AU - Dickmeis, Thomas
AU - Peravali, Ravindra
AU - Rastegar, Sepand
AU - Strähle, Uwe
AU - Hsiao, I. Lun
AU - Gilliland, Douglas
AU - Ojea-Jimenez, Isaac
AU - Ambrose, Selina V.Y.
AU - Belinga-Desaunay-Nault, Marie France A.
AU - Khan, Abdullah O.
AU - Lynch, Iseult
AU - Valsami-Jones, Eugenia
AU - Diabaté, Silvia
AU - Weiss, Carsten
N1 - Funding Information:
This research was funded by the European Commission's 7th Framework Program (FP7/2007-2013) research project NanoMILE (Contract No. NMP4-LA-2013-310451). We thank Nicholas Foulkes for helpful comments on the manuscript and Katrin Volkmann for technical assistance.
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2022/1
Y1 - 2022/1
N2 - The growing number of manufactured nanomaterials (MNMs) used in consumer products and industrial applications is leading to increased exposures whose consequences are not yet fully understood in terms of potential impacts on human and ecosystem health. Thus, there is an urgent need to further develop high throughput testing for hazard prediction in nanotoxicology. The zebrafish (Danio rerio) embryo has emerged as a vertebrate model organism for nanotoxicity studies, as it provides superior characteristics for microscopy-based screening and can describe the complex interactions of MNMs with a living organism. Automated microscopy coupled with image acquisition has facilitated hazard assessment of chemicals by quantification of various endpoints (e.g., mortality, malformations, hatching), an approach which we employed here to address adverse effects of a selected library of MNMs of different compositions and surface functionalities. We investigated metal and metal oxide MNMs with mean primary particle sizes of 5 to 50 nm, different chemical compositions (silica, ceria, titania, zinc oxide and silver) and various surface coatings/functionalisation, including OECD reference materials. In addition, we tested as representatives of nanoplastics polystyrene and polymethylmethacrylate nanoparticles which were surface modified by either amino- or carboxyl-groups. Our systematic analysis to establish quantitative property-activity relationships revealed a pronounced toxicity of silver and amino-modified polystyrene nanoparticles (NPs), evidenced by reduced survival and malformations. Interference with hatching, however, was the most sensitive endpoint, as zinc oxide as well as silver NPs reduced hatching already at lower concentrations (i.e., 0.5 and 1 μg mL-1, respectively) and early time points (3 days post fertilization, dpf). Interestingly, carboxylated polystyrene NPs and carboxylated or amino-modified polymethylmethacrylate NPs were non-toxic, highlighting both surface modification and core chemistry of nanoplastics as key determinants of biocompatibility. This journal is
AB - The growing number of manufactured nanomaterials (MNMs) used in consumer products and industrial applications is leading to increased exposures whose consequences are not yet fully understood in terms of potential impacts on human and ecosystem health. Thus, there is an urgent need to further develop high throughput testing for hazard prediction in nanotoxicology. The zebrafish (Danio rerio) embryo has emerged as a vertebrate model organism for nanotoxicity studies, as it provides superior characteristics for microscopy-based screening and can describe the complex interactions of MNMs with a living organism. Automated microscopy coupled with image acquisition has facilitated hazard assessment of chemicals by quantification of various endpoints (e.g., mortality, malformations, hatching), an approach which we employed here to address adverse effects of a selected library of MNMs of different compositions and surface functionalities. We investigated metal and metal oxide MNMs with mean primary particle sizes of 5 to 50 nm, different chemical compositions (silica, ceria, titania, zinc oxide and silver) and various surface coatings/functionalisation, including OECD reference materials. In addition, we tested as representatives of nanoplastics polystyrene and polymethylmethacrylate nanoparticles which were surface modified by either amino- or carboxyl-groups. Our systematic analysis to establish quantitative property-activity relationships revealed a pronounced toxicity of silver and amino-modified polystyrene nanoparticles (NPs), evidenced by reduced survival and malformations. Interference with hatching, however, was the most sensitive endpoint, as zinc oxide as well as silver NPs reduced hatching already at lower concentrations (i.e., 0.5 and 1 μg mL-1, respectively) and early time points (3 days post fertilization, dpf). Interestingly, carboxylated polystyrene NPs and carboxylated or amino-modified polymethylmethacrylate NPs were non-toxic, highlighting both surface modification and core chemistry of nanoplastics as key determinants of biocompatibility. This journal is
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U2 - 10.1039/d1en00299f
DO - 10.1039/d1en00299f
M3 - Article
AN - SCOPUS:85123960319
SN - 2051-8153
VL - 9
SP - 375
EP - 392
JO - Environmental Science: Nano
JF - Environmental Science: Nano
IS - 1
ER -
