Abstract
Imperatorin is a naturally occurring furocoumarin compound isolated from plants such as Angelica archangelica and Cnidium monnieri. It has multiple pharmacological effects including anticonvulsant effects. Here we determined the effects of imperatorin on voltage-gated Na+ channels (VGSC) using whole-cell patch clamp techniques in differentiated neuronal NG108-15 cells. We showed that imperatorin inhibited VGSC; such inhibition did not show state-dependence. Imperatorin caused a left shift in the steady-state inactivation curve without affecting activation gating. The inhibition of VGSC by imperatorin displayed a mild frequency-dependence. Imperatorin was also shown to inhibit VGSC and action potential amplitude without affecting voltage-gated K+ channels in rat hippocampal CA1 neurons. In conclusion, our results suggest that imperatorin dampens neuronal excitability by inhibiting VGSC.
Original language | English |
---|---|
Pages (from-to) | 49-55 |
Number of pages | 7 |
Journal | European Journal of Pharmacology |
Volume | 721 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - Dec 5 2013 |
Keywords
- Hippocampal CA1 neurons
- Imperatorin
- NG108-15 cells
- Voltage-gated Na channels
ASJC Scopus subject areas
- Pharmacology