Suppression of isoproterenol-induced apoptosis in H9c2 cardiomyoblast cells by daidzein through activation of Akt

Wei Syun Hu, Yueh Min Lin, Wei Wen Kuo, Lung Fa Pan, Yu Lan Yeh, Yi Hui Li, Chia Hua Kuo, Ray Jade Chen, V. Vijaya Padma, Tung Sheng Chen, Chih Yang Huang

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Increased serum norepinephrine level is one of pathological processes relating to heart disease (HD). Estrogens are considered as potential therapeutics for the treatment of HD; however, estrogen supplementation shows some side-effects, such as increasing the risk of developing breast, endometrial and ovarian cancers. This study investigated the cardio-protective effects of daidzein (Dai), a selective estrogen receptor modulator (SERM) from soy bean extract, in H9c2 cardiomyoblast cells treated with isoproterenol (ISO), a norepinephrine analog. In this in vitro model, H9c2 cells treated with Dai at different concentrations showed no statistical difference in cell viability. TdT-mediated digoxigenin-dUTP nick-end labeling (TUNEL) data and western blotting results indicated that Dai treated-H9c2 cells recovered from the damage induced by ISO. The recovery effects of Dai on ISO-induced damage were blocked by inhibition of Akt activation through adding Akt inhibitor. On the other hand, the fold changes of phosphorylated Akt (p-Akt)/Akt normalized with the control for con, 0.25, 0.5, 1, 3 and 24 h of treatment were 1, 2, 5, 13, 11 and 10, respectively. In conclusion, Dai ameliorates apoptosis of cardiomyoblasts induced by ISO through Akt signaling pathway.

Original languageEnglish
Pages (from-to)323-330
Number of pages8
JournalChinese Journal of Physiology
Issue number6
Publication statusPublished - 2016


  • Apoptosis
  • Daidzein
  • Estrogen
  • Heart disease
  • Isoproterenol

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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