Suppression of cell growth by heavy chain ferritin

Jia Hsin Guo; Shu Hui Juan; Steven D. Aust

doi:10.1006/bbrc.1997.7910

Suppression of cell growth by heavy chain ferritin

Jia Hsin Guo, Shu Hui Juan, Steven D. Aust

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.

Original language	English
Pages (from-to)	39-45
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	242
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1997.7910
Publication status	Published - Jan 6 1998
Externally published	Yes

Keywords

Cell suppression
Iron
Recombinant ferritin

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.1997.7910

Cite this

@article{4fde15fdc14046cc99452d2ec13bd6b5,

title = "Suppression of cell growth by heavy chain ferritin",

abstract = "While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.",

keywords = "Cell suppression, Iron, Recombinant ferritin",

author = "Guo, {Jia Hsin} and Juan, {Shu Hui} and Aust, {Steven D.}",

note = "Funding Information: We thank Majid Abedi for construction of the T7 expression system using the M13 vector, Thomas B. Cardon and G. Ryan Berger for excellent technical assistance, and T. Maughan for secretarial assistance in preparation of the manuscript. This work was supported in part by NIH Grant ES05056.",

year = "1998",

month = jan,

day = "6",

doi = "10.1006/bbrc.1997.7910",

language = "English",

volume = "242",

pages = "39--45",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Suppression of cell growth by heavy chain ferritin

AU - Guo, Jia Hsin

AU - Juan, Shu Hui

AU - Aust, Steven D.

N1 - Funding Information: We thank Majid Abedi for construction of the T7 expression system using the M13 vector, Thomas B. Cardon and G. Ryan Berger for excellent technical assistance, and T. Maughan for secretarial assistance in preparation of the manuscript. This work was supported in part by NIH Grant ES05056.

PY - 1998/1/6

Y1 - 1998/1/6

N2 - While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.

AB - While producing recombinant rat liver H and L chain ferritin homopolymers using the baculovirus expression system, we noticed that rat liver H chain ferritin, but not L chain ferritin, had a suppressive effect on the growth of Spodoptera frugiperda (Sf-21) cells. Suppression was observed immediately after infection with recombinant H chain ferritin baculovirus prepared from lysed infected cells. Immediate suppression was observed when purified with either recombinant H chain apoferritin or various holoferritins (loaded with 1970 ± 50 or 2520 ± 90 atoms of iron/ ferritin) indicating that suppression was not due to sequestration of iron required for cell growth. Suppression by H chain ferritin was also observed upon attempting to express the protein in Escherichia coli. Strategies for expression of recombinant rat liver H and L ferritin homopolymers in both prokaryotic and eukaryotic expression systems were developed.

KW - Cell suppression

KW - Iron

KW - Recombinant ferritin

UR - http://www.scopus.com/inward/record.url?scp=0032488542&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032488542&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1997.7910

DO - 10.1006/bbrc.1997.7910

M3 - Article

C2 - 9439606

AN - SCOPUS:0032488542

SN - 0006-291X

VL - 242

SP - 39

EP - 45

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Suppression of cell growth by heavy chain ferritin

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this