Abstract
Objective - Expression of adhesion molecules on endothelial cells and subsequent leukocyte recruitment are critical early events in the development of atherosclerosis. We tried to study possible effects of Cu/Zn superoxide dismutase (SOD) on adhesion molecule expression and its underlying mechanism in the prevention and treatment of cardiovascular disorders. Methods and Results - Human aortic endothelial cells (HAECs) were transfected with adenovirus carrying the human SOD gene (AdSOD) to investigate whether SOD expression in HAECs attenuated tumor necrosis factor (TNF)-α-induced reactive oxygen species production and adhesion molecule expression and to define the mechanisms involved. SOD expression significantly suppressed TNF-α-induced expression of vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1 and reduced the binding of the human neutrophils to TNF-α-stimulated HAECs. SOD expression suppressed c-JUN N-terminal kinase and p38 phosphorylation. It also attenuated intracellular superoxide anion production and NADPH oxidase activity in TNF-α-treated HAECs. Conclusions - These results provide evidence that SOD expression in endothelial cells attenuates TNF-α-induced superoxide anion production and adhesion molecule expression, and that this protective effect is mediated by decreased JNK and p38 phosphorylation and activator protein-1 and nuclear factor κB inactivation. These results suggest that SOD has antiinflammatory properties and may play important roles in the prevention of atherosclerosis and inflammatory response.
Original language | English |
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Pages (from-to) | 334-340 |
Number of pages | 7 |
Journal | Arteriosclerosis, Thrombosis, and Vascular Biology |
Volume | 25 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 2005 |
Externally published | Yes |
Keywords
- Adhesion molecule
- Atherosclerosis
- Endothelial cell
- MAPKs
- Superoxide dismutase
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine