TY - JOUR
T1 - Superoxide anion scavenge effect of querem glauca thunb. in whole blood of patients with ankylosing spondylitis
AU - Sheu, Shiow Yunn
AU - Tsuang, Yang Hwei
AU - Hsu, Feng Lin
AU - Lu, Fung Jou
AU - Chiang, Hsiich Ching
PY - 1997
Y1 - 1997
N2 - Nine phenolic compounds, catechin (1), epicatechin (2), gallocatechin (3), epigallocatechin (4), procyanidin B-4 (5), catechin-3-O-rhamnoside (6), rutin (7), querglanin (8) and isoquerglanin (9) were isolated from oak leaves (Quercus glauca Thunb. Fagaceae). the latter two (8, 9) were identified as new compounds. Several Quercus species have been used in folk medicine as an astringent for hemorrhoids and for treatment of inflammation, jaundice. tumor. In this study, these compounds were tested for scavenging effects of the Superoxide anion in the whole blood of patients with ankylosing spondylitis by means of an ultra-sensitive chemoluminescence (CL) analyzer and lucigenin amplification. The results showed that at a concentration of 2.3 × 10-5 M, isoquerglanin (9) displayed the strongest inhibition activity (73.55%), followed by querglanin (8) (68.81 %) and then gallocatechin (3) and epigallocatechin (4) (66.97 and 60.17% inhibition, respectively). In addition, the blood chemoluminescence (CL) level of patients with ankylosing spondylitis was inhibited by Superoxide dismutase (SOD) but not by catalase, suggesting that Superoxide anion is the major component of reactive oxygen species (ROS) involved in this assay system.
AB - Nine phenolic compounds, catechin (1), epicatechin (2), gallocatechin (3), epigallocatechin (4), procyanidin B-4 (5), catechin-3-O-rhamnoside (6), rutin (7), querglanin (8) and isoquerglanin (9) were isolated from oak leaves (Quercus glauca Thunb. Fagaceae). the latter two (8, 9) were identified as new compounds. Several Quercus species have been used in folk medicine as an astringent for hemorrhoids and for treatment of inflammation, jaundice. tumor. In this study, these compounds were tested for scavenging effects of the Superoxide anion in the whole blood of patients with ankylosing spondylitis by means of an ultra-sensitive chemoluminescence (CL) analyzer and lucigenin amplification. The results showed that at a concentration of 2.3 × 10-5 M, isoquerglanin (9) displayed the strongest inhibition activity (73.55%), followed by querglanin (8) (68.81 %) and then gallocatechin (3) and epigallocatechin (4) (66.97 and 60.17% inhibition, respectively). In addition, the blood chemoluminescence (CL) level of patients with ankylosing spondylitis was inhibited by Superoxide dismutase (SOD) but not by catalase, suggesting that Superoxide anion is the major component of reactive oxygen species (ROS) involved in this assay system.
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M3 - Article
C2 - 9358904
AN - SCOPUS:0031308139
SN - 0192-415X
VL - 25
SP - 307
EP - 315
JO - American Journal of Chinese Medicine
JF - American Journal of Chinese Medicine
IS - 3-4
ER -