SUMO modification modulates the transrepression activity of PLZF

Ting Ting Chao; Che Chang Chang; Hsiu Ming Shih

doi:10.1016/j.bbrc.2007.04.157

SUMO modification modulates the transrepression activity of PLZF

Ting Ting Chao, Che Chang Chang, Hsiu Ming Shih

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of PML sumoylation in PML nuclear body formation. Furthermore, PLZF-3KR markedly reduced the transcriptional repression activity, correlating with a loss of PLZF-mediated growth suppression. These results reveal an important role of SUMO modification in PLZF-mediated transcriptional repression.

Original language	English
Pages (from-to)	475-482
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	358
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2007.04.157
Publication status	Published - Jun 29 2007
Externally published	Yes

Keywords

Nuclear body
PLZF
SUMO modification
Transcriptional repression

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2007.04.157

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title = "SUMO modification modulates the transrepression activity of PLZF",

abstract = "Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of PML sumoylation in PML nuclear body formation. Furthermore, PLZF-3KR markedly reduced the transcriptional repression activity, correlating with a loss of PLZF-mediated growth suppression. These results reveal an important role of SUMO modification in PLZF-mediated transcriptional repression.",

keywords = "Nuclear body, PLZF, SUMO modification, Transcriptional repression",

author = "Chao, {Ting Ting} and Chang, {Che Chang} and Shih, {Hsiu Ming}",

note = "Funding Information: We thank Drs. Ronald Evans, Daniel Tenen, and Huey-Kang Sytwu for the pCMX-HA-PLZF, c-myc-Luc and pTY-EF1α constructs, respectively. This work was supported by Extramural Funds of National Health Research Institutes (NHRI-EX95-9529NI), NSC95-2311-B-001-027 and thematic research program of Academia Sinica (AS-95-TP-B02) to H.-M.S. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2007",

month = jun,

day = "29",

doi = "10.1016/j.bbrc.2007.04.157",

language = "English",

volume = "358",

pages = "475--482",

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T1 - SUMO modification modulates the transrepression activity of PLZF

AU - Chao, Ting Ting

AU - Chang, Che Chang

AU - Shih, Hsiu Ming

N1 - Funding Information: We thank Drs. Ronald Evans, Daniel Tenen, and Huey-Kang Sytwu for the pCMX-HA-PLZF, c-myc-Luc and pTY-EF1α constructs, respectively. This work was supported by Extramural Funds of National Health Research Institutes (NHRI-EX95-9529NI), NSC95-2311-B-001-027 and thematic research program of Academia Sinica (AS-95-TP-B02) to H.-M.S. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2007/6/29

Y1 - 2007/6/29

N2 - Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of PML sumoylation in PML nuclear body formation. Furthermore, PLZF-3KR markedly reduced the transcriptional repression activity, correlating with a loss of PLZF-mediated growth suppression. These results reveal an important role of SUMO modification in PLZF-mediated transcriptional repression.

AB - Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of PML sumoylation in PML nuclear body formation. Furthermore, PLZF-3KR markedly reduced the transcriptional repression activity, correlating with a loss of PLZF-mediated growth suppression. These results reveal an important role of SUMO modification in PLZF-mediated transcriptional repression.

KW - Nuclear body

KW - PLZF

KW - SUMO modification

KW - Transcriptional repression

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SUMO modification modulates the transrepression activity of PLZF

Abstract

Keywords

ASJC Scopus subject areas

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