Sulforaphane suppressed LPS-induced inflammation in mouse peritoneal macrophages through Nrf2 dependent pathway

Wen Lin, Rachel T. Wu, Tienyuan Wu, Tin Oo Khor, Hu Wang, Ah Ng Kong

Research output: Contribution to journalArticlepeer-review

282 Citations (Scopus)

Abstract

Sulforaphane (SFN) is a natural isothiocyanate that is present in cruciferous vegetables such as broccoli and cabbage. Previous studies have shown that SFN is effective in preventing carcinogenesis induced by carcinogens in rodents, which is related in part to its potent anti-inflammation properties. In the present study, we compared the anti-inflammatory effect of SFN on LPS-stimulated inflammation in primary peritoneal macrophages derived from Nrf2 (+/+) and Nrf2 (-/-) mice. Pretreatment of SFN in Nrf2 (+/+) primary peritoneal macrophages potently inhibited LPS-stimulated mRNA expression, protein expression and production of TNF-α, IL-1β, COX-2 and iNOS. HO-1 expression was significantly augmented in LPS-stimulated Nrf2 (+/+) primary peritoneal macrophages by SFN. Interestingly, the anti-inflammatory effect was attenuated in Nrf2 (-/-) primary peritoneal macrophages. We concluded that SFN exerts its anti-inflammatory activity mainly via activation of Nrf2 in mouse peritoneal macrophages.

Original languageEnglish
Pages (from-to)967-973
Number of pages7
JournalBiochemical Pharmacology
Volume76
Issue number8
DOIs
Publication statusPublished - Oct 2008
Externally publishedYes

Keywords

  • Cyclooxygenase-2
  • Inducible nitric oxide synthase
  • Inflammation
  • Interleukin-1β
  • Nuclear E2-factor related factor 2
  • Peritoneal macrophage
  • Sulforaphane
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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