Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein

Matthew M. Skinner; Hong Zhang; Dale H. Leschnitzer; Yue Guan; Henry Bellamy; Robert M. Sweet; Carla W. Gray; Ruud N.H. Konings; Andrew H.J. Wang; Thomas C. Terwilliger

doi:10.1073/pnas.91.6.2071

Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein

Matthew M. Skinner, Hong Zhang, Dale H. Leschnitzer, Yue Guan, Henry Bellamy, Robert M. Sweet, Carla W. Gray, Ruud N.H. Konings, Andrew H.J. Wang, Thomas C. Terwilliger

Research output: Contribution to journal › Article › peer-review

116 Citations (Scopus)

Abstract

The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47 → methionine mutant proteins with x-ray diffraction data phased to 2.5 Å resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 Å resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein- single-stranded DNA complex.

Original language	English
Pages (from-to)	2071-2075
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	6
DOIs	https://doi.org/10.1073/pnas.91.6.2071
Publication status	Published - Mar 15 1994
Externally published	Yes

Keywords

DNA binding protein
protein structure
protein-DNA complex
synchrotron radiation
x-ray crystallography

ASJC Scopus subject areas

General

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10.1073/pnas.91.6.2071

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Skinner, M. M., Zhang, H., Leschnitzer, D. H., Guan, Y., Bellamy, H., Sweet, R. M., Gray, C. W., Konings, R. N. H., Wang, A. H. J., & Terwilliger, T. C. (1994). Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein. Proceedings of the National Academy of Sciences of the United States of America, 91(6), 2071-2075. https://doi.org/10.1073/pnas.91.6.2071

Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein. / Skinner, Matthew M.; Zhang, Hong; Leschnitzer, Dale H. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 6, 15.03.1994, p. 2071-2075.

Research output: Contribution to journal › Article › peer-review

Skinner, MM, Zhang, H, Leschnitzer, DH, Guan, Y, Bellamy, H, Sweet, RM, Gray, CW, Konings, RNH, Wang, AHJ & Terwilliger, TC 1994, 'Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein', Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 6, pp. 2071-2075. https://doi.org/10.1073/pnas.91.6.2071

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title = "Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein",

abstract = "The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47 → methionine mutant proteins with x-ray diffraction data phased to 2.5 {\AA} resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 {\AA} resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein- single-stranded DNA complex.",

keywords = "DNA binding protein, protein structure, protein-DNA complex, synchrotron radiation, x-ray crystallography",

author = "Skinner, {Matthew M.} and Hong Zhang and Leschnitzer, {Dale H.} and Yue Guan and Henry Bellamy and Sweet, {Robert M.} and Gray, {Carla W.} and Konings, {Ruud N.H.} and Wang, {Andrew H.J.} and Terwilliger, {Thomas C.}",

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doi = "10.1073/pnas.91.6.2071",

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AU - Skinner, Matthew M.

AU - Zhang, Hong

AU - Leschnitzer, Dale H.

AU - Guan, Yue

AU - Bellamy, Henry

AU - Sweet, Robert M.

AU - Gray, Carla W.

AU - Konings, Ruud N.H.

AU - Wang, Andrew H.J.

AU - Terwilliger, Thomas C.

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KW - DNA binding protein

KW - protein structure

KW - protein-DNA complex

KW - synchrotron radiation

KW - x-ray crystallography

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Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein

Abstract

Keywords

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