Structural Basis for Stabilization of Z-DNA by Cobalt Hexaammine and Magnesium Cations

Reinhard V. Gessner; Gary J. Quigley; Andrew H.J. Wang; Alexander Rich; Gijs A. van der Marel; Jacques H. van Boom

doi:10.1021/bi00323a001

Structural Basis for Stabilization of Z-DNA by Cobalt Hexaammine and Magnesium Cations

Reinhard V. Gessner, Gary J. Quigley, Andrew H.J. Wang, Alexander Rich, Gijs A. van der Marel, Jacques H. van Boom

Research output: Contribution to journal › Article › peer-review

226 Citations (Scopus)

Abstract

In the equilibrium between B-DNA and Z-DNA in poly(dC-dG), the [Co(NH₃)₆]³⁺ ion stabilizes the Z form 4 orders of magnitude more effectively than the Mg²⁺ ion. The structural basis of this difference is revealed in Z-DNA crystal structures of d(CpGpCpGpCpG) stabilized by either Na⁺/Mg²⁺ or Na⁺/Mg²⁺ plus [Co(NH₃)₆]³⁺. The crystals diffract X-rays to high resolution, and the structures were refined at 1.25 A. The [Co(NH₃)₆]³⁺ ion forms five hydrogen bonds onto the surface of Z-DNA, bonding to a guanine O6 and N7 as well as to a phosphate group in the Zn conformation. The Mg²⁺ ion binds through its hydration shell with up to three hydrogen bonds to guanine N7 and O6. Higher charge, specific fitting of more hydrogen bonds, and a more stable complex all contribute to the great effectiveness of [Co(NH₃)₆]³⁺ in stabilizing Z-DNA.

Original language	English
Pages (from-to)	237-240
Number of pages	4
Journal	Biochemistry
Volume	24
Issue number	2
DOIs	https://doi.org/10.1021/bi00323a001
Publication status	Published - Jan 1 1985
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

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title = "Structural Basis for Stabilization of Z-DNA by Cobalt Hexaammine and Magnesium Cations",

abstract = "In the equilibrium between B-DNA and Z-DNA in poly(dC-dG), the [Co(NH3)6]3+ ion stabilizes the Z form 4 orders of magnitude more effectively than the Mg2+ ion. The structural basis of this difference is revealed in Z-DNA crystal structures of d(CpGpCpGpCpG) stabilized by either Na+/Mg2+ or Na+/Mg2+ plus [Co(NH3)6]3+. The crystals diffract X-rays to high resolution, and the structures were refined at 1.25 A. The [Co(NH3)6]3+ ion forms five hydrogen bonds onto the surface of Z-DNA, bonding to a guanine O6 and N7 as well as to a phosphate group in the Zn conformation. The Mg2+ ion binds through its hydration shell with up to three hydrogen bonds to guanine N7 and O6. Higher charge, specific fitting of more hydrogen bonds, and a more stable complex all contribute to the great effectiveness of [Co(NH3)6]3+ in stabilizing Z-DNA.",

author = "Gessner, \{Reinhard V.\} and Quigley, \{Gary J.\} and Wang, \{Andrew H.J.\} and Alexander Rich and \{van der Marel\}, \{Gijs A.\} and \{van Boom\}, \{Jacques H.\}",

year = "1985",

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doi = "10.1021/bi00323a001",

language = "English",

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journal = "Biochemistry",

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T1 - Structural Basis for Stabilization of Z-DNA by Cobalt Hexaammine and Magnesium Cations

AU - Gessner, Reinhard V.

AU - Quigley, Gary J.

AU - Wang, Andrew H.J.

AU - Rich, Alexander

AU - van der Marel, Gijs A.

AU - van Boom, Jacques H.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - In the equilibrium between B-DNA and Z-DNA in poly(dC-dG), the [Co(NH3)6]3+ ion stabilizes the Z form 4 orders of magnitude more effectively than the Mg2+ ion. The structural basis of this difference is revealed in Z-DNA crystal structures of d(CpGpCpGpCpG) stabilized by either Na+/Mg2+ or Na+/Mg2+ plus [Co(NH3)6]3+. The crystals diffract X-rays to high resolution, and the structures were refined at 1.25 A. The [Co(NH3)6]3+ ion forms five hydrogen bonds onto the surface of Z-DNA, bonding to a guanine O6 and N7 as well as to a phosphate group in the Zn conformation. The Mg2+ ion binds through its hydration shell with up to three hydrogen bonds to guanine N7 and O6. Higher charge, specific fitting of more hydrogen bonds, and a more stable complex all contribute to the great effectiveness of [Co(NH3)6]3+ in stabilizing Z-DNA.

AB - In the equilibrium between B-DNA and Z-DNA in poly(dC-dG), the [Co(NH3)6]3+ ion stabilizes the Z form 4 orders of magnitude more effectively than the Mg2+ ion. The structural basis of this difference is revealed in Z-DNA crystal structures of d(CpGpCpGpCpG) stabilized by either Na+/Mg2+ or Na+/Mg2+ plus [Co(NH3)6]3+. The crystals diffract X-rays to high resolution, and the structures were refined at 1.25 A. The [Co(NH3)6]3+ ion forms five hydrogen bonds onto the surface of Z-DNA, bonding to a guanine O6 and N7 as well as to a phosphate group in the Zn conformation. The Mg2+ ion binds through its hydration shell with up to three hydrogen bonds to guanine N7 and O6. Higher charge, specific fitting of more hydrogen bonds, and a more stable complex all contribute to the great effectiveness of [Co(NH3)6]3+ in stabilizing Z-DNA.

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Structural Basis for Stabilization of Z-DNA by Cobalt Hexaammine and Magnesium Cations

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