Stimulatory effect of D-ephedrine on β3-adrenoceptors in adipose tissue of rats

Juei Tang Cheng; I. Min Liu; Shi Ting Yen; Shiow Wen Juang; Tsang Pai Liu; Paul Chan

doi:10.1016/S1566-0702(01)00225-9

Stimulatory effect of D-ephedrine on β₃-adrenoceptors in adipose tissue of rats

Juei Tang Cheng, I. Min Liu, Shi Ting Yen, Shiow Wen Juang, Tsang Pai Liu, Paul Chan

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The effect of ephedrine on β₃-adrenoceptors (β₃-AR) was studied in the isolated adipose tissue of Wistar rat. Incubation with D-ephedrine (0.1-10 μM) induced a concentration-dependent decrease of uptake of [¹⁴C]-deoxy-D-glucose into white adipose tissues (WAT). The inhibitory effect of D-ephedrine was potentiated by BRL 37344, the agonist of β₃-AR and concentration-dependently inhibited by SR 59230A, the selective antagonist of β₃-AR. The action of D-ephedrine on β₃-AR was further blocked by the antibodies for β₃-AR, but not the immunoglobulin, in a concentration-dependent manner. Moreover, D-ephedrine increased glycerol release from the isolated brown adipose tissues (BAT) and this action was also abolished by SR 59230A at concentration sufficient to block β₃-AR. Thus, these results suggest that D-ephedrine has the ability to activate β₃-AR both in WAT and BAT of Wistar rats in vitro.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	Autonomic Neuroscience: Basic and Clinical
Volume	88
Issue number	1-2
DOIs	https://doi.org/10.1016/S1566-0702(01)00225-9
Publication status	Published - Apr 12 2001

Keywords

BRL 37344
Brown adipose tissues
D-Ephedrine
Glucose uptake
Lipolysis
SR 59230A
White adipose tissues
β-Adrenoceptors

ASJC Scopus subject areas

Endocrine and Autonomic Systems
Clinical Neurology
Cellular and Molecular Neuroscience

Access to Document

10.1016/S1566-0702(01)00225-9

Cite this

@article{39bf913292b647f1ad32c482661b8117,

title = "Stimulatory effect of D-ephedrine on β3-adrenoceptors in adipose tissue of rats",

abstract = "The effect of ephedrine on β3-adrenoceptors (β3-AR) was studied in the isolated adipose tissue of Wistar rat. Incubation with D-ephedrine (0.1-10 μM) induced a concentration-dependent decrease of uptake of [14C]-deoxy-D-glucose into white adipose tissues (WAT). The inhibitory effect of D-ephedrine was potentiated by BRL 37344, the agonist of β3-AR and concentration-dependently inhibited by SR 59230A, the selective antagonist of β3-AR. The action of D-ephedrine on β3-AR was further blocked by the antibodies for β3-AR, but not the immunoglobulin, in a concentration-dependent manner. Moreover, D-ephedrine increased glycerol release from the isolated brown adipose tissues (BAT) and this action was also abolished by SR 59230A at concentration sufficient to block β3-AR. Thus, these results suggest that D-ephedrine has the ability to activate β3-AR both in WAT and BAT of Wistar rats in vitro.",

keywords = "BRL 37344, Brown adipose tissues, D-Ephedrine, Glucose uptake, Lipolysis, SR 59230A, White adipose tissues, β-Adrenoceptors",

author = "Cheng, {Juei Tang} and Liu, {I. Min} and Yen, {Shi Ting} and Juang, {Shiow Wen} and Liu, {Tsang Pai} and Paul Chan",

note = "Funding Information: We appreciate Dr. Luciano Manara (Sanofi Midy, Milan, Italy) for kindly supplying SR 59230A. Thanks are also due to Taiwan Green Cross (Taipei, Taiwan) for the donation of IVIG and Chong Dah Pharmaceuticals (Tainan, Taiwan) for the supply of l -ephedrine and Professor Y.C. Tong for editing. We thank Prof. David C.Y. Kwan of McMaster University for his help in the preparation of the final version of this manuscript. This work is supported in part by a grant from National Science Council (NSC87-2314-B006-104) of the Republic of China.",

year = "2001",

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TY - JOUR

T1 - Stimulatory effect of D-ephedrine on β3-adrenoceptors in adipose tissue of rats

AU - Cheng, Juei Tang

AU - Liu, I. Min

AU - Yen, Shi Ting

AU - Juang, Shiow Wen

AU - Liu, Tsang Pai

AU - Chan, Paul

N1 - Funding Information: We appreciate Dr. Luciano Manara (Sanofi Midy, Milan, Italy) for kindly supplying SR 59230A. Thanks are also due to Taiwan Green Cross (Taipei, Taiwan) for the donation of IVIG and Chong Dah Pharmaceuticals (Tainan, Taiwan) for the supply of l -ephedrine and Professor Y.C. Tong for editing. We thank Prof. David C.Y. Kwan of McMaster University for his help in the preparation of the final version of this manuscript. This work is supported in part by a grant from National Science Council (NSC87-2314-B006-104) of the Republic of China.

PY - 2001/4/12

Y1 - 2001/4/12

N2 - The effect of ephedrine on β3-adrenoceptors (β3-AR) was studied in the isolated adipose tissue of Wistar rat. Incubation with D-ephedrine (0.1-10 μM) induced a concentration-dependent decrease of uptake of [14C]-deoxy-D-glucose into white adipose tissues (WAT). The inhibitory effect of D-ephedrine was potentiated by BRL 37344, the agonist of β3-AR and concentration-dependently inhibited by SR 59230A, the selective antagonist of β3-AR. The action of D-ephedrine on β3-AR was further blocked by the antibodies for β3-AR, but not the immunoglobulin, in a concentration-dependent manner. Moreover, D-ephedrine increased glycerol release from the isolated brown adipose tissues (BAT) and this action was also abolished by SR 59230A at concentration sufficient to block β3-AR. Thus, these results suggest that D-ephedrine has the ability to activate β3-AR both in WAT and BAT of Wistar rats in vitro.

AB - The effect of ephedrine on β3-adrenoceptors (β3-AR) was studied in the isolated adipose tissue of Wistar rat. Incubation with D-ephedrine (0.1-10 μM) induced a concentration-dependent decrease of uptake of [14C]-deoxy-D-glucose into white adipose tissues (WAT). The inhibitory effect of D-ephedrine was potentiated by BRL 37344, the agonist of β3-AR and concentration-dependently inhibited by SR 59230A, the selective antagonist of β3-AR. The action of D-ephedrine on β3-AR was further blocked by the antibodies for β3-AR, but not the immunoglobulin, in a concentration-dependent manner. Moreover, D-ephedrine increased glycerol release from the isolated brown adipose tissues (BAT) and this action was also abolished by SR 59230A at concentration sufficient to block β3-AR. Thus, these results suggest that D-ephedrine has the ability to activate β3-AR both in WAT and BAT of Wistar rats in vitro.

KW - BRL 37344

KW - Brown adipose tissues

KW - D-Ephedrine

KW - Glucose uptake

KW - Lipolysis

KW - SR 59230A

KW - White adipose tissues

KW - β-Adrenoceptors

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U2 - 10.1016/S1566-0702(01)00225-9

DO - 10.1016/S1566-0702(01)00225-9

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SN - 1566-0702

VL - 88

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JO - Autonomic Neuroscience: Basic and Clinical

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