TY - JOUR
T1 - Stimulation of prostaglandin cyclooxygenase and prostacyclin synthetase activities by estradiol in rat aortic smooth muscle cells
AU - Wen-Chang, Chang
AU - Junko, Nakao
AU - Hajime, Orimo
AU - Sei-Itsu, Murota
N1 - Funding Information:
The authors wish to thank Ono Pharmaceutical Co., Ltd., Osaka, Japan for its financial support to W.C.C. and for the generous gifts of standard prostaglandins and thromboxane Bz. Thanks are also due to Dr. Ikuo Morita for his help in gas-liquid chromatographic analyses and to Dr. George S. Roth for his critical reading of this manuscript.
PY - 1980/12/5
Y1 - 1980/12/5
N2 - The effects of estradiol on the arachidonic acid pool and prostacyclin biosynthetic activity in rat aortic smooth muscle cells were studied. Estradiol has no significant effect on the distribution of [14C] arachidonic acid in cells with respect to prostacyclin production assay, the endogenous fatty acid (specifically, arachidonic acid) composition of cellular phospholipid fractions and cellular phospholipase (or/and lipase) activities. However, estradiol significantly stimulates both prostaglandin cyclooxygenase and prostacyclin synthetase activities of cells, and induction of new protein biosynthesis is involved in the effect of estradiol on the stimulation of prostacyclin biosynthetic activity.
AB - The effects of estradiol on the arachidonic acid pool and prostacyclin biosynthetic activity in rat aortic smooth muscle cells were studied. Estradiol has no significant effect on the distribution of [14C] arachidonic acid in cells with respect to prostacyclin production assay, the endogenous fatty acid (specifically, arachidonic acid) composition of cellular phospholipid fractions and cellular phospholipase (or/and lipase) activities. However, estradiol significantly stimulates both prostaglandin cyclooxygenase and prostacyclin synthetase activities of cells, and induction of new protein biosynthesis is involved in the effect of estradiol on the stimulation of prostacyclin biosynthetic activity.
KW - (Rat aortic smooth muscle cell)
KW - Atherosclerosis
KW - Estradiol
KW - Prostacyclin synthetase
KW - Prostaglandin cyclooxygenase
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U2 - 10.1016/0005-2760(80)90139-3
DO - 10.1016/0005-2760(80)90139-3
M3 - Article
C2 - 6786344
AN - SCOPUS:0019158169
SN - 0005-2760
VL - 620
SP - 472
EP - 482
JO - BBA - Specialised Section On Lipids and Related Subjects
JF - BBA - Specialised Section On Lipids and Related Subjects
IS - 3
ER -