Stimulation of 12-lipoxygenase activity in rat platelets by 17β-estradiol

Wen Chang Chang; Junko Nakao; Hajime Orimo; Hsin Hsiung Tai; Sei Itsu Murota

doi:10.1016/0006-2952(82)90710-9

Stimulation of 12-lipoxygenase activity in rat platelets by 17β-estradiol

Wen Chang Chang, Junko Nakao, Hajime Orimo, Hsin Hsiung Tai, Sei Itsu Murota

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The effects of estradiol on the endogenous fatty acid (specifically, arachidonic acid) composition of cellular phospholipid fractions and the 12-lipoxygenase activity in rat platelets in vivo were studied. Estradiol had no significant effect on the endogenous fatty acid composition of cellular phospholipid fractions. However, estradiol significantly increased 12-lipoxygenase activity in platelets in a dose-dependent manner. The stimulatory effect of estradiol on platelet lipoxygenase was blocked by the anti-estrogen nafoxidine hydrochloride which was injected simultaneously together with estradiol in vivo, suggesting that the effect in target cells was due directly to estradiol.

Original language	English
Pages (from-to)	2633-2638
Number of pages	6
Journal	Biochemical Pharmacology
Volume	31
Issue number	16
DOIs	https://doi.org/10.1016/0006-2952(82)90710-9
Publication status	Published - Aug 15 1982
Externally published	Yes

Keywords

12-HPETE
12-l-hydoxy-5,8,10,14-eicosatetraenoic acid
12-l-hydroperoxy-5,8,10,14-eicosatetraenoic acid
12-l-hydroxy-5,8,10-heptadecatrienoic acid
2-HETE
GSH
HHT
and GLC
gas-liquid chromtography
reduced glugatione

ASJC Scopus subject areas

Biochemistry
Pharmacology

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10.1016/0006-2952(82)90710-9

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title = "Stimulation of 12-lipoxygenase activity in rat platelets by 17β-estradiol",

abstract = "The effects of estradiol on the endogenous fatty acid (specifically, arachidonic acid) composition of cellular phospholipid fractions and the 12-lipoxygenase activity in rat platelets in vivo were studied. Estradiol had no significant effect on the endogenous fatty acid composition of cellular phospholipid fractions. However, estradiol significantly increased 12-lipoxygenase activity in platelets in a dose-dependent manner. The stimulatory effect of estradiol on platelet lipoxygenase was blocked by the anti-estrogen nafoxidine hydrochloride which was injected simultaneously together with estradiol in vivo, suggesting that the effect in target cells was due directly to estradiol.",

keywords = "12-HPETE, 12-l-hydoxy-5,8,10,14-eicosatetraenoic acid, 12-l-hydroperoxy-5,8,10,14-eicosatetraenoic acid, 12-l-hydroxy-5,8,10-heptadecatrienoic acid, 2-HETE, GSH, HHT, and GLC, gas-liquid chromtography, reduced glugatione",

author = "Chang, {Wen Chang} and Junko Nakao and Hajime Orimo and Tai, {Hsin Hsiung} and Murota, {Sei Itsu}",

year = "1982",

month = aug,

day = "15",

doi = "10.1016/0006-2952(82)90710-9",

language = "English",

volume = "31",

pages = "2633--2638",

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issn = "0006-2952",

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TY - JOUR

T1 - Stimulation of 12-lipoxygenase activity in rat platelets by 17β-estradiol

AU - Chang, Wen Chang

AU - Nakao, Junko

AU - Orimo, Hajime

AU - Tai, Hsin Hsiung

AU - Murota, Sei Itsu

PY - 1982/8/15

Y1 - 1982/8/15

N2 - The effects of estradiol on the endogenous fatty acid (specifically, arachidonic acid) composition of cellular phospholipid fractions and the 12-lipoxygenase activity in rat platelets in vivo were studied. Estradiol had no significant effect on the endogenous fatty acid composition of cellular phospholipid fractions. However, estradiol significantly increased 12-lipoxygenase activity in platelets in a dose-dependent manner. The stimulatory effect of estradiol on platelet lipoxygenase was blocked by the anti-estrogen nafoxidine hydrochloride which was injected simultaneously together with estradiol in vivo, suggesting that the effect in target cells was due directly to estradiol.

AB - The effects of estradiol on the endogenous fatty acid (specifically, arachidonic acid) composition of cellular phospholipid fractions and the 12-lipoxygenase activity in rat platelets in vivo were studied. Estradiol had no significant effect on the endogenous fatty acid composition of cellular phospholipid fractions. However, estradiol significantly increased 12-lipoxygenase activity in platelets in a dose-dependent manner. The stimulatory effect of estradiol on platelet lipoxygenase was blocked by the anti-estrogen nafoxidine hydrochloride which was injected simultaneously together with estradiol in vivo, suggesting that the effect in target cells was due directly to estradiol.

KW - 12-HPETE

KW - 12-l-hydoxy-5,8,10,14-eicosatetraenoic acid

KW - 12-l-hydroperoxy-5,8,10,14-eicosatetraenoic acid

KW - 12-l-hydroxy-5,8,10-heptadecatrienoic acid

KW - 2-HETE

KW - GSH

KW - HHT

KW - and GLC

KW - gas-liquid chromtography

KW - reduced glugatione

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UR - http://www.scopus.com/inward/citedby.url?scp=0019971270&partnerID=8YFLogxK

U2 - 10.1016/0006-2952(82)90710-9

DO - 10.1016/0006-2952(82)90710-9

M3 - Article

C2 - 6814440

AN - SCOPUS:0019971270

SN - 0006-2952

VL - 31

SP - 2633

EP - 2638

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 16

ER -

Stimulation of 12-lipoxygenase activity in rat platelets by 17β-estradiol

Abstract

Keywords

ASJC Scopus subject areas

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