Abstract
Objective To determine the content of sperm mitochondrial DNA (mtDNA) in patients with asthenospermia or with poor sperm motility. Design Analysis of the content of mtDNA as the ratio between the amount of mtDNA and nuclear DNA by using a new concurrent polymerase chain reaction. Setting University hospital infertility center. Patient(s) Eighty-six men who sought infertility therapy. Intervention(s) Moving characteristics of sperm were examined with a computer-assisted semen analyzer. Main outcome measure(s) Sperm samples were classified into two groups, one with asthenospermia and the other with normal moving characteristics. The content of mtDNA in sperm was determined by polymerase chain reaction. We analyzed the mitochondrial mass by MitoTracker Green staining and analyzed DNA content with propidium iodide staining by flow cytometry. Result(s) A decrease in sperm mtDNA content was detected in patients with asthenospermia or with poor sperm motility (
| Original language | English |
|---|---|
| Pages (from-to) | 66-73 |
| Number of pages | 8 |
| Journal | Fertility and Sterility |
| Volume | 82 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 2004 |
Keywords
- Depletion
- infertility
- mitochondrial DNA
- motility
- sperm
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Reproductive Medicine