Size of Micelles Determined by Static Fluorescence Quenching

We developed a new method to measure the average aggregation number of large rod‐like micelles using static fluorescence self‐quenching of a solubilized fluorophore. The method is based on the increase of self‐quenching of micelle‐solubilized pyrene through excimer formation. We consider the effect of random distribution of pyrene in micelles and the micellar size distribution. The measured average aggregation <n> _M is based on a new M‐weighted raging similar to our exponential‐weighted averaging in the transient decay method. We apply this method to study the effect of a large concentration of salt on the average aggregation behavior of sodium dodecyl sulfate (SDS) and cetyle tetraammonium bromide (CTAB). The sizes increase with increasing ionic concentrations. For SDS, we used the thermodynamic model developed by Missel et al. to calculate < n > _M which we compare with experimental results.