TY - JOUR
T1 - Single-layer periosteum progenitor cell sheet significantly promotes tendon-bone healing in comparison with acellular collagen sheet in anterior cruciate ligament reconstruction
AU - Chen, Lei Yen
AU - Whu, Shu Wen
AU - Chen, Chih Hwa
AU - Liu, Hsia Wei
AU - Liu, Hsien Tao
AU - Chang, Chih Hsiang
AU - Tsai, Ching Lin
AU - Chen, Shih Hui
AU - Hsiue, Ging Ho
N1 - Funding Information:
The authors would like to thank the National Science Council (grant NSC96-2314-B-182A-054-MY3 and 099-2811-B-182-014 ) and Chang Gung Memorial Hospital (grant CMRPG-280331 ) for financial support of this research. The authors also acknowledge the staff of the Medical Research Center, the Laboratory Animal Center, and the Confocal Laser Scanning Microscopic Room at Chang Gung Memorial Hospital–Keelung for their technical assistance.
PY - 2012/2
Y1 - 2012/2
N2 - Purpose: Successful tendon-bone healing in anterior cruciate ligament reconstruction can be achieved by introducing a suitable medium into the tendon-bone junction. Two new feasible media, periosteum progenitor cell sheet (PPCS) and acellular collagen sheet (ACS), were utilized to promote tendon-bone healing. This study aimed to compare the effects of these two media on tendon-bone healing in a rabbit model. Methods: Periosteum progenitor cells were harvested from rabbit tibial periosteum, cultivated on polymerized fibrin-coated dishes, and obtained as PPCS. ACS was prepared from rabbit small intestinal submucosa. Twenty rabbits were used in this study. Tendon grafts wrapped in cell sheet were pulled into a bone tunnel in a model of anterior cruciate ligament reconstruction. Rabbits were sacrificed at 8 weeks postoperatively for histological, immunohistochemical, and biomechanical assay. Results: The monolayer PPCS that had been wrapped around the tendon before surgery was analysed. Histological staining revealed that PPCS gave rise to higher collagen and glycosaminoglycan deposition with fibrocartilage formation than ACS at the tendon-bone junction at 8 weeks. In addition, PPCS produced significantly better mechanical results compared with ACS, including increased maximum load, stiffness, and maximum load per length of bone tunnel. Conclusions: Single-layer PPCS significantly enhanced tendon-bone healing compared with ACS, with fibrocartilage formation at the tendon interface and superior biomechanical strength.
AB - Purpose: Successful tendon-bone healing in anterior cruciate ligament reconstruction can be achieved by introducing a suitable medium into the tendon-bone junction. Two new feasible media, periosteum progenitor cell sheet (PPCS) and acellular collagen sheet (ACS), were utilized to promote tendon-bone healing. This study aimed to compare the effects of these two media on tendon-bone healing in a rabbit model. Methods: Periosteum progenitor cells were harvested from rabbit tibial periosteum, cultivated on polymerized fibrin-coated dishes, and obtained as PPCS. ACS was prepared from rabbit small intestinal submucosa. Twenty rabbits were used in this study. Tendon grafts wrapped in cell sheet were pulled into a bone tunnel in a model of anterior cruciate ligament reconstruction. Rabbits were sacrificed at 8 weeks postoperatively for histological, immunohistochemical, and biomechanical assay. Results: The monolayer PPCS that had been wrapped around the tendon before surgery was analysed. Histological staining revealed that PPCS gave rise to higher collagen and glycosaminoglycan deposition with fibrocartilage formation than ACS at the tendon-bone junction at 8 weeks. In addition, PPCS produced significantly better mechanical results compared with ACS, including increased maximum load, stiffness, and maximum load per length of bone tunnel. Conclusions: Single-layer PPCS significantly enhanced tendon-bone healing compared with ACS, with fibrocartilage formation at the tendon interface and superior biomechanical strength.
KW - Anterior cruciate ligament reconstruction
KW - Periosteum progenitor cell sheet
KW - Tendon bone healing
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U2 - 10.1016/j.fjmd.2012.01.001
DO - 10.1016/j.fjmd.2012.01.001
M3 - Article
AN - SCOPUS:84862788940
SN - 2210-7940
VL - 3
SP - 1
EP - 8
JO - Formosan Journal of Musculoskeletal Disorders
JF - Formosan Journal of Musculoskeletal Disorders
IS - 1
ER -