TY - JOUR
T1 - Simpor leaf extract- (Dillenia suffruticosa Martelli) induced apoptosis of the MCF-7 and HepG2 cell lines
AU - Rahayu, Sri
AU - Chang, Jungshan
AU - Supiyani, Atin
AU - Prasetiyo, Arief
N1 - Publisher Copyright:
© 2023 Sri Rahayu et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
PY - 2023/5
Y1 - 2023/5
N2 - Cancer is a malignant tumor with characteristics of uncontrolled cell growth, poor metastasis, leading to the dysfunction of organs and death of patients. Many efforts have been made, including the exploration of natural products. The purpose of this study was to determine the cytotoxicity of the Dillenia suffruticosa leaf extract against the cell lines MCF-7 and HepG2. The method used is an experimental research design with a completely randomized design. The treatment group consisted of the leaf extract of 500, 1,000, 2,000, 5,000, and 9,000 ppm for the brine shrimp lethality test (BSLT). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test used 10, 15, 20, 25, and 50 ppm concentrations and control (without extract). The data on the BSLT test was determined by analyzing the lethal concentration 50 (LC50) value using the probit analysis, while the MTT test was determined by analyzing the IC50 and analysis of variance test. The BSLT test results showed an LC50 value of 5,221 ppm, which means that the leaf extract is not toxic. Simpor leaf extract at 25 ppm with IC50 of 88.52 was seen as the best concentration for liver cancer treatment. It can be concluded that D. suffruticosa shows cytotoxicity against the cell lines MCF-7 and HepG2 and can be used as a candidate for breast and liver cancer treatment.
AB - Cancer is a malignant tumor with characteristics of uncontrolled cell growth, poor metastasis, leading to the dysfunction of organs and death of patients. Many efforts have been made, including the exploration of natural products. The purpose of this study was to determine the cytotoxicity of the Dillenia suffruticosa leaf extract against the cell lines MCF-7 and HepG2. The method used is an experimental research design with a completely randomized design. The treatment group consisted of the leaf extract of 500, 1,000, 2,000, 5,000, and 9,000 ppm for the brine shrimp lethality test (BSLT). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test used 10, 15, 20, 25, and 50 ppm concentrations and control (without extract). The data on the BSLT test was determined by analyzing the lethal concentration 50 (LC50) value using the probit analysis, while the MTT test was determined by analyzing the IC50 and analysis of variance test. The BSLT test results showed an LC50 value of 5,221 ppm, which means that the leaf extract is not toxic. Simpor leaf extract at 25 ppm with IC50 of 88.52 was seen as the best concentration for liver cancer treatment. It can be concluded that D. suffruticosa shows cytotoxicity against the cell lines MCF-7 and HepG2 and can be used as a candidate for breast and liver cancer treatment.
KW - BSLT
KW - Cytotoxicity
KW - HepG2 cell line
KW - leaf
KW - MCF-7 cell line
KW - simpor Dillenia
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U2 - 10.7324/JAPS.2023.121327
DO - 10.7324/JAPS.2023.121327
M3 - Article
AN - SCOPUS:85159168070
SN - 2231-3354
VL - 13
SP - 107
EP - 113
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
IS - 5
ER -
