TY - JOUR
T1 - Sesamol induces nitric oxide release from human umbilical vein endothelial cells
AU - Chen, Pey Rong
AU - Tsai, Chingmin E.
AU - Chang, Hang
AU - Liu, Tsuei Ling
AU - Lee, Chun Chung
N1 - Funding Information:
aDepartment of Nutrition and Food Science, Fu Jen Catholic University, Taipei, Taiwan, bDepartment of Dietetics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, cDepartment of Bioscience Technology, Chung Yuan University, Taoyan, Taiwan, dDepartment of Medical Education and Research, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, and eDepartment of Pharmacy, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
Funding Information:
*To whom correspondence should be addressed at 161, Sec. 6, Min-Chuan East Road, 7F of national Defense Medical Center, division of Cancer Research, National Health Research Institutes, Taipei 114, Taiwan. E-mail: [email protected] or [email protected] Abbreviations: Akt, protein kinase B; EC, endothelial cell; ECE-1, endothelin-converting enzyme-1; ECL, enhanced chemiluminescence; EDRF, endothelium-derived relaxing factor; EIA, enzyme-linked immunosorbent assay; eNOS (= NOS3), endothelial NO synthase; ET-1, endothelin-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HUVEC, human umbilical vein endothelial cells; L-NAME, Nω-nitro-L-arginine methyl ester; NO, nitric oxide; NOS, nitric oxide synthase; PA, plasminogen activator; PI-3K, PI-3 kinase; PVDF, polyvinylidene difluoride; ROS, reactive oxygen species; RT, reverse transcription; SSC, saline-sodium citrate; TBST, Tris-buffered saline Tween-20; tPA, tissue plasminogen activator; uPA urokinase plasminogen activator; VSMC, vascular smooth muscle cells.
PY - 2005/9
Y1 - 2005/9
N2 - Sesamol, which is derived from sesame seed lignans, is reportedly an antioxidant. Nitric oxide (NO), the most important vascular relaxing factor, is regulated in the endothelium. In addition, NO is involved in protecting endothelium and has antiatherosclerotic and antithrombotic activities. The endothelium produces NO through the regulation of both endothelial NO synthase (eNOS) expression and activity in endothelial cells. This study sought to investigate the effect of sesamol on NO released from human umbilical vein endothelial cells (HUVEC) and to examine the expression and activity of eNOS. Sesamol induced NO release from endothelial cells in a dose-dependent manner (from 1 to 10 μM), as measured 24 h after treatment; the expression of the eNOS gene at both transcription and translation levels; and NOS activity in endothelial cells. The content of cGMP was also increased by sesamol through NO signaling. The transcription of eNOS induced by sesamol was confirmed through the activation of PI-3 kinase-Akt (protein kinase B) signaling. The results demonstrate that sesamol induces NOS signaling pathways in HUVEC and suggest a role for sesamol in cardiovascular reactivity In vivo.
AB - Sesamol, which is derived from sesame seed lignans, is reportedly an antioxidant. Nitric oxide (NO), the most important vascular relaxing factor, is regulated in the endothelium. In addition, NO is involved in protecting endothelium and has antiatherosclerotic and antithrombotic activities. The endothelium produces NO through the regulation of both endothelial NO synthase (eNOS) expression and activity in endothelial cells. This study sought to investigate the effect of sesamol on NO released from human umbilical vein endothelial cells (HUVEC) and to examine the expression and activity of eNOS. Sesamol induced NO release from endothelial cells in a dose-dependent manner (from 1 to 10 μM), as measured 24 h after treatment; the expression of the eNOS gene at both transcription and translation levels; and NOS activity in endothelial cells. The content of cGMP was also increased by sesamol through NO signaling. The transcription of eNOS induced by sesamol was confirmed through the activation of PI-3 kinase-Akt (protein kinase B) signaling. The results demonstrate that sesamol induces NOS signaling pathways in HUVEC and suggest a role for sesamol in cardiovascular reactivity In vivo.
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U2 - 10.1007/s11745-005-1456-3
DO - 10.1007/s11745-005-1456-3
M3 - Article
C2 - 16329468
AN - SCOPUS:27444441555
SN - 0024-4201
VL - 40
SP - 955
EP - 961
JO - Lipids
JF - Lipids
IS - 9
M1 - L9718
ER -