TY - JOUR
T1 - Sequence conservation among the cognate nonstructural NS3/3A protein genes of six bluetongue viruses
AU - Guang-Yuh, Hwang
AU - Yi-Yuan, Yang
AU - Jwo-Farn, Chiou
AU - Joseph, K. K.Li
N1 - Funding Information:
We express our sincere thanks to Ms. Mary Wise, and Mr. Russell Mayes for assistance in cell culture, virus propagation and purification, to Ms. Lynnette Harris for manuscript preparation and to Ms. Cindy Peterson for typing of the manuscript. This study was supported partly by USDA Grant 90-37266-5567,t he Utah Agricultural Experiment Station Project 537 and Utah Biotechnology Grant 4-20195 to J.K.-K. Li and is approved as Journal Paper 4567. J.-F. Chiou was supported by a NTUMC fellowship from National Taiwan University.
PY - 1992/4
Y1 - 1992/4
N2 - Full-length cDNA copies of segment 10 genes of bluetongue virus serotypes 2, 11, 13 and 17 were synthesized by the Clamp-R method and inserted into the plasmid pUC19. The complete nucleotide sequences of these four cognate genes were sequenced and determined to be 822 nucleotides in length, smallest of the 10 genes in the bluetongue virion. These four cognate gene segments contained two in-phase and overlapping open reading frames capable of coding for two non-structural proteins of 229 and 216 amino acids with net charges of + 4.5 and + 5.5, respectively, at neutral pH. Comparative analyses of the predicted amino acid sequences of bluetongue virus serotypes 1, 2, 10, 11, 13 and 17 revealed (i) a high degree of sequence homology and conservation, (ii) a single conserved tryptophan located at residue 159, (iii) the presence of two conserved cysteines at residues 137 and 181 and two potential N-linked glycosylation sites at residues 63-65 and 150-152, (iv) a cluster of six prolines within a 15-amino acid region near the amino terminus, and (v) the longest 3' noncoding sequence of 113 bases among the 10 bluetongue viral genes. Phylogenetic analyses indicated that BTV-10 and -11 are very closely related and BTV-2 is the distantly related serotype of the five US bluetongue virus serotypes.
AB - Full-length cDNA copies of segment 10 genes of bluetongue virus serotypes 2, 11, 13 and 17 were synthesized by the Clamp-R method and inserted into the plasmid pUC19. The complete nucleotide sequences of these four cognate genes were sequenced and determined to be 822 nucleotides in length, smallest of the 10 genes in the bluetongue virion. These four cognate gene segments contained two in-phase and overlapping open reading frames capable of coding for two non-structural proteins of 229 and 216 amino acids with net charges of + 4.5 and + 5.5, respectively, at neutral pH. Comparative analyses of the predicted amino acid sequences of bluetongue virus serotypes 1, 2, 10, 11, 13 and 17 revealed (i) a high degree of sequence homology and conservation, (ii) a single conserved tryptophan located at residue 159, (iii) the presence of two conserved cysteines at residues 137 and 181 and two potential N-linked glycosylation sites at residues 63-65 and 150-152, (iv) a cluster of six prolines within a 15-amino acid region near the amino terminus, and (v) the longest 3' noncoding sequence of 113 bases among the 10 bluetongue viral genes. Phylogenetic analyses indicated that BTV-10 and -11 are very closely related and BTV-2 is the distantly related serotype of the five US bluetongue virus serotypes.
KW - Bluetongue virus
KW - Cognate gene
KW - Molecular phylogeny
KW - Nucleotide sequence
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U2 - 10.1016/0168-1702(92)90074-J
DO - 10.1016/0168-1702(92)90074-J
M3 - Article
C2 - 1318624
AN - SCOPUS:0026536159
SN - 0168-1702
VL - 23
SP - 151
EP - 161
JO - Virus Research
JF - Virus Research
IS - 1-2
ER -
