Separation and structure determination of nicardipine photoproducts by LC-ESI-MS

Shih Ming Chen; Mau Chih Hsieh; Su Hui Chao; E-E Chang; Po Yu Wang; An-Bang Wu

doi:10.1002/bmc.1020

Separation and structure determination of nicardipine photoproducts by LC-ESI-MS

Shih Ming Chen, Mau Chih Hsieh, Su Hui Chao, E-E Chang, Po Yu Wang, An-Bang Wu

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A sample of 0.104 M nicardipine in methanol was photoirradiated with a Philips 400 W UV lamp for 3 h in a photochemical chamber. A total of four major photoproducts were found from the HPLC chromatogram. The same sample was used for taking LC-MS, while eight major photoproducts were observed and the structures elucidated by analyzing the CID patterns of their respective mass spectra. A reaction scheme of nicardipine is proposed that the photochemical reactions occur mainly via oxidation of 1,4-dihydropyridine moiety, following the stepwise photo-reduction of the m-nitro group and demethylation of the ester group at 5-position of the pyridine ring.

Original language	English
Pages (from-to)	1008-1012
Number of pages	5
Journal	Biomedical Chromatography
Volume	22
Issue number	9
DOIs	https://doi.org/10.1002/bmc.1020
Publication status	Published - Sept 2008

Keywords

HPLC
LC-MS
Nicardipine
Photoproducts

ASJC Scopus subject areas

Drug Discovery
Analytical Chemistry
Molecular Biology
Biochemistry
Clinical Biochemistry
Pharmacology

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10.1002/bmc.1020

Cite this

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title = "Separation and structure determination of nicardipine photoproducts by LC-ESI-MS",

abstract = "A sample of 0.104 M nicardipine in methanol was photoirradiated with a Philips 400 W UV lamp for 3 h in a photochemical chamber. A total of four major photoproducts were found from the HPLC chromatogram. The same sample was used for taking LC-MS, while eight major photoproducts were observed and the structures elucidated by analyzing the CID patterns of their respective mass spectra. A reaction scheme of nicardipine is proposed that the photochemical reactions occur mainly via oxidation of 1,4-dihydropyridine moiety, following the stepwise photo-reduction of the m-nitro group and demethylation of the ester group at 5-position of the pyridine ring.",

keywords = "HPLC, LC-MS, Nicardipine, Photoproducts",

author = "Chen, {Shih Ming} and Hsieh, {Mau Chih} and Chao, {Su Hui} and E-E Chang and Wang, {Po Yu} and An-Bang Wu",

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T1 - Separation and structure determination of nicardipine photoproducts by LC-ESI-MS

AU - Chen, Shih Ming

AU - Hsieh, Mau Chih

AU - Chao, Su Hui

AU - Chang, E-E

AU - Wang, Po Yu

AU - Wu, An-Bang

PY - 2008/9

Y1 - 2008/9

N2 - A sample of 0.104 M nicardipine in methanol was photoirradiated with a Philips 400 W UV lamp for 3 h in a photochemical chamber. A total of four major photoproducts were found from the HPLC chromatogram. The same sample was used for taking LC-MS, while eight major photoproducts were observed and the structures elucidated by analyzing the CID patterns of their respective mass spectra. A reaction scheme of nicardipine is proposed that the photochemical reactions occur mainly via oxidation of 1,4-dihydropyridine moiety, following the stepwise photo-reduction of the m-nitro group and demethylation of the ester group at 5-position of the pyridine ring.

AB - A sample of 0.104 M nicardipine in methanol was photoirradiated with a Philips 400 W UV lamp for 3 h in a photochemical chamber. A total of four major photoproducts were found from the HPLC chromatogram. The same sample was used for taking LC-MS, while eight major photoproducts were observed and the structures elucidated by analyzing the CID patterns of their respective mass spectra. A reaction scheme of nicardipine is proposed that the photochemical reactions occur mainly via oxidation of 1,4-dihydropyridine moiety, following the stepwise photo-reduction of the m-nitro group and demethylation of the ester group at 5-position of the pyridine ring.

KW - HPLC

KW - LC-MS

KW - Nicardipine

KW - Photoproducts

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SN - 0269-3879

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JO - Biomedical Chromatography

JF - Biomedical Chromatography

IS - 9

ER -

Separation and structure determination of nicardipine photoproducts by LC-ESI-MS

Abstract

Keywords

ASJC Scopus subject areas

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