Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis

Sz Hsien Yu; Pei Ming Yang; Chih Wen Peng; Yi Chu Yu; Shu Jun Chiu

doi:10.1016/j.canlet.2010.09.015

Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis

Sz Hsien Yu, Pei Ming Yang, Chih Wen Peng, Yi Chu Yu, Shu Jun Chiu

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.

Original language	English
Pages (from-to)	96-104
Number of pages	9
Journal	Cancer Letters
Volume	300
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2010.09.015
Publication status	Published - Jan 1 2011

Keywords

Fisetin
Human colon cancer cells
P53
Securin

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2010.09.015

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title = "Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis",

abstract = "Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.",

keywords = "Fisetin, Human colon cancer cells, P53, Securin",

author = "Yu, {Sz Hsien} and Yang, {Pei Ming} and Peng, {Chih Wen} and Yu, {Yi Chu} and Chiu, {Shu Jun}",

year = "2011",

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doi = "10.1016/j.canlet.2010.09.015",

language = "English",

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AU - Yu, Sz Hsien

AU - Yang, Pei Ming

AU - Peng, Chih Wen

AU - Yu, Yi Chu

AU - Chiu, Shu Jun

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.

AB - Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.

KW - Fisetin

KW - Human colon cancer cells

KW - P53

KW - Securin

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Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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