TY - JOUR
T1 - Screening rat mesenchymal stem cell attachment and differentiation on surface chemistries using plasma polymer gradients
AU - Wang, Peng Yuan
AU - Clements, Lauren R.
AU - Thissend, Helmut
AU - Tsaia, Wei Bor
AU - Voelckere, Nicolas H.
N1 - Funding Information:
The authors gratefully acknowledge funding from the Australian Research Council. P.Y.W. acknowledges support from the National Science Council (Taiwan) via the award of a travel scholarship under the Graduate Program for Studying (GPS) in Australia/New Zealand scheme (98-2911-I-002-056). L.C. acknowledges support from the CSIRO Office of the Chief Executive (OCE). We are grateful to Marta Redrado Notivoli from the Materials Characterisation and Fabrication Platform, University of Melbourne, for her assistance with AFM measurements.
Publisher Copyright:
© 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
PY - 2015
Y1 - 2015
N2 - It is well known that the surface chemistry of biomaterials is important for both initial cell attachment and the downstream cell response. Surface chemistry gradients are a new format that allows the screening of the subtleties of cell-surface interactions in high throughput. In this study, two surface chemical gradients were fabricated using diffusion control during plasma polymerization via a tilted mask. Acrylic acid (AA) plasma polymer gradients were coated on a uniform 1,7-octadiene (OD) plasma polymer layer to generate OD-AA plasma polymer gradients, whilst diethylene glycol dimethyl ether (DG) plasma polymer gradients were coated on a uniform AA plasma polymer layer to generate AA-DG plasma polymer gradients. Gradient surfaces were characterized by X-ray photoelectron spectroscopy, infrared microscopy mapping, profilometry, water contact angle (WCA) goniometry and atomic force microscopy. Cell attachment density and differentiation into osteo- and adipo-lineages of rat-bone-marrow mesenchymal stem cells (rBMSCs) was studied on gradients. Cell adhesion after 24 h culture was sensitive to the chemical gradients, resulting in a cell density gradient along the substrate. The slope of the cell density gradient changed between 24 and 6 days due to cell migration and growth. Induction of rBMSCs into osteoblast- and adipocyte-like cells on the two plasma polymer gradients suggested that osteogenic differentiation was sensitive to local cell density, but adipogenic differentiation was not. Using mixed induction medium (50% osteogenic and 50% adipogenic medium), thick AA plasma polymer coating (>40 nm thickness with ∼11% COOH component and 35° WCA) robustly supported osteogenic differentiation as determined by colony formation and calcium deposition. This study establishes a simple but powerful approach to the formation of plasma polymer based gradients, and demonstrates that MSC behavior can be influenced by small changes in surface chemistry.
AB - It is well known that the surface chemistry of biomaterials is important for both initial cell attachment and the downstream cell response. Surface chemistry gradients are a new format that allows the screening of the subtleties of cell-surface interactions in high throughput. In this study, two surface chemical gradients were fabricated using diffusion control during plasma polymerization via a tilted mask. Acrylic acid (AA) plasma polymer gradients were coated on a uniform 1,7-octadiene (OD) plasma polymer layer to generate OD-AA plasma polymer gradients, whilst diethylene glycol dimethyl ether (DG) plasma polymer gradients were coated on a uniform AA plasma polymer layer to generate AA-DG plasma polymer gradients. Gradient surfaces were characterized by X-ray photoelectron spectroscopy, infrared microscopy mapping, profilometry, water contact angle (WCA) goniometry and atomic force microscopy. Cell attachment density and differentiation into osteo- and adipo-lineages of rat-bone-marrow mesenchymal stem cells (rBMSCs) was studied on gradients. Cell adhesion after 24 h culture was sensitive to the chemical gradients, resulting in a cell density gradient along the substrate. The slope of the cell density gradient changed between 24 and 6 days due to cell migration and growth. Induction of rBMSCs into osteoblast- and adipocyte-like cells on the two plasma polymer gradients suggested that osteogenic differentiation was sensitive to local cell density, but adipogenic differentiation was not. Using mixed induction medium (50% osteogenic and 50% adipogenic medium), thick AA plasma polymer coating (>40 nm thickness with ∼11% COOH component and 35° WCA) robustly supported osteogenic differentiation as determined by colony formation and calcium deposition. This study establishes a simple but powerful approach to the formation of plasma polymer based gradients, and demonstrates that MSC behavior can be influenced by small changes in surface chemistry.
KW - Differentiation
KW - Mesenchymal stem cells
KW - Plasma polymers
KW - Surface chemistry
KW - Surface-bound gradients
UR - http://www.scopus.com/inward/record.url?scp=84924902135&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84924902135&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2014.09.027
DO - 10.1016/j.actbio.2014.09.027
M3 - Article
C2 - 25246312
AN - SCOPUS:84924902135
SN - 1742-7061
VL - 11
SP - 58
EP - 67
JO - Acta Biomaterialia
JF - Acta Biomaterialia
IS - 1
ER -