@article{28ef9f6e15be4298824b246ba955ed12,
title = "Role of calcium ions in the positive interaction between TRPA1 and TRPV1 channels in bronchopulmonary sensory neurons",
abstract = "Both transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are abundantly expressed in bronchopulmonary C-fiber sensory nerves and can be activated by a number of endogenous inflammatory mediators. A recent study has reported a synergistic effect of simultaneous TRPA1 and TRPV1 activations in vagal pulmonary C-fiber afferents in anesthetized rats, but its underlying mechanism was not known. This study aimed to characterize a possible interaction between these two TRP channels and to investigate the potential role of Ca2+ as a mediator of this interaction in isolated rat vagal pulmonary sensory neurons. Using the perforated patch-clamp recording technique, our study demonstrated a distinct positive interaction occurring abruptly between TRPA1 and TRPV1 when they were activated simultaneously by their respective agonists, capsaicin (Cap) and allyl isothiocyanate (AITC), at near-threshold concentrations in these neurons. AITC at this low concentration evoked only minimal or undetectable responses, but it markedly amplified the Cap-evoked current in the same neurons. This potentiating effect was eliminated when either AITC or Cap was replaced by non-TRPA1 and non-TRPV1 chemical activators of these neurons, demonstrating the selectivity of the interaction between these two TRP channels. Furthermore, when Ca2+ was removed from the extracellular solution, the synergistic effect of Cap and AITC on pulmonary sensory neurons was completely abrogated, clearly indicating a critical role of Ca2+ in mediating the action. These results suggest that this TRPA1-TRPV1 interaction may play a part in regulating the sensitivity of pulmonary sensory neurons during airway inflammatory reaction. Copyright {\textcopyright} 2015 the American Physiological Society.",
keywords = "Airway, Inflammation, Sensory nerve, Transient receptor potential ankyrin 1, Transient receptor potential vanilloid 1",
author = "Chun-Chun Hsu and Lu-Yuan Lee",
note = "被引用次數：1 Export Date: 11 May 2016 CODEN: JAPHE 通訊地址: Lee, L.-Y.; Department of Physiology, University of Kentucky Medical CenterUnited States 出資詳情: HL-96914, NIH, National Institutes of Health 出資詳情: UL1TR0000117, NIH, National Institutes of Health 參考文獻: Ahern, G.P., Brooks, I.M., Miyares, R.L., Wang, X.B., Extracellular cations sensitize and gate capsaicin receptor TRPV1 modulating pain signaling (2005) J Neurosci, 25, pp. 5109-5116; Akopian, A.N., Regulation of nociceptive transmission at the periphery via TRPA1-TRPV1 interactions (2011) Curr Pharm Biotechnol, 12, pp. 89-94; Bandell, M., Story, G.M., Hwang, S.W., Viswanath, V., Eid, S.R., Petrus, M.J., Earley, T.J., Patapoutian, A., Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin (2004) Neuron, 41, pp. 849-857; Bautista, D.M., Jordt, S.E., Nikai, T., Tsuruda, P.R., Read, A.J., Poblete, J., Yamoah, E.N., Julius, D., TRPA1 mediates the inflammatory actions of environmental irritants and proalgesic agents (2006) Cell, 124, pp. 1269-1282; 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year = "2015",
doi = "10.1152/japplphysiol.00043.2015",
language = "English",
volume = "118",
pages = "1533--1543",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "12",
}