TY - JOUR
T1 - Riboflavin protects mice against liposaccharide-induced shock through expression of heat shock protein 25
AU - Shih, Chun Kuang
AU - Chen, Chiao-Ming
AU - Chen, C. Y Oliver
AU - Liu, Jen-Fang
AU - Lin, Hui Wen
AU - Chou, Hung Tsung
AU - Li, Sing Chung
N1 - Funding Information:
This study was supported by a research Grant NSC 94-2320-B-038-044 from the National Science Council and sponsored by the Taipei Medical University–Wan Fang Hospital (94TMU-WFH-218), Taiwan, ROC. We also thank research assistant Ms. Chia-Wen Chou and Yung-Ching Chan for the work.
PY - 2010/7
Y1 - 2010/7
N2 - Riboflavin (vitamin B2) is a water-soluble vitamin essential for normal cellular functions, growth and development. This study aimed to investigate the effects of vitamin B2 on the survival rate, and expressions of tissue heat shock protein 25 (HSP25) and heat shock factor 1 (HSF1) in mice undergoing lipopolysaccharide (LPS) induced shock. Mice were assigned to four groups, saline vehicle, LPS, LPS plus low dose of vitamin B2 (LB2) and LPS plus high dose of vitamin B2 (HB2). Vitamin B2 (1 and 10. mg/kg BW) was administered intraperitoneally at 2 and 0. h before the i.p. administration of LPS. At the end of the experiment, the survival rate monitored was 10, 20, 60, and 100% for LPS, LB2, HB2, and saline mice, respectively. HSP25 expressions in the heart and lung were significantly enhanced in a time-dependent manner in the HB2 mice as compared to the saline mice (p<0.05), but not altered in the LB2 mice. In the HB2 mice, plasma riboflavin concentrations reached 300. nM at 6. h post LPS and returned to the 0. h level at 72. h. The results showed that high dose of riboflavin could decrease LPS-induced mortality through an increased expression of HSP25.
AB - Riboflavin (vitamin B2) is a water-soluble vitamin essential for normal cellular functions, growth and development. This study aimed to investigate the effects of vitamin B2 on the survival rate, and expressions of tissue heat shock protein 25 (HSP25) and heat shock factor 1 (HSF1) in mice undergoing lipopolysaccharide (LPS) induced shock. Mice were assigned to four groups, saline vehicle, LPS, LPS plus low dose of vitamin B2 (LB2) and LPS plus high dose of vitamin B2 (HB2). Vitamin B2 (1 and 10. mg/kg BW) was administered intraperitoneally at 2 and 0. h before the i.p. administration of LPS. At the end of the experiment, the survival rate monitored was 10, 20, 60, and 100% for LPS, LB2, HB2, and saline mice, respectively. HSP25 expressions in the heart and lung were significantly enhanced in a time-dependent manner in the HB2 mice as compared to the saline mice (p<0.05), but not altered in the LB2 mice. In the HB2 mice, plasma riboflavin concentrations reached 300. nM at 6. h post LPS and returned to the 0. h level at 72. h. The results showed that high dose of riboflavin could decrease LPS-induced mortality through an increased expression of HSP25.
KW - Heat shock factor 1
KW - Heat shock protein 25
KW - Lipopolysaccharide
KW - Sepsis
KW - Vitamin B2
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U2 - 10.1016/j.fct.2010.04.033
DO - 10.1016/j.fct.2010.04.033
M3 - Article
C2 - 20430062
AN - SCOPUS:77953535875
SN - 0278-6915
VL - 48
SP - 1913
EP - 1918
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 7
ER -