Riboflavin at High Doses Enhances Lung Cancer Cell Proliferation, Invasion, and Migration

Hui Ting Yang, Pei Chun Chao, Mei Chin Yin

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


The influence of riboflavin (vitamin B2) upon growth, invasion, and migration in non-small cell lung cancer cell lines was evaluated. Riboflavin at 1, 10, 25, 50, 100, 200, or 400 μmol/L was added into A549, H3255, or Calu-6 cells. The effects of this compound upon level and/or expression of reactive oxygen species (ROS), inflammatory cytokines, intercellular adhesion molecule (ICAM)-1, fibronectin, matrix metalloproteinase (MMP)-9, MMP-2, focal adhesion kinase (FAK), nuclear factor kappa B (NF-κB), and mitogen-activated protein kinase (MAPK) were examined. Results showed that riboflavin at test doses did not affect the level of ROS and glutathione. Riboflavin at 200 and 400 μmol/L significantly enhanced cell growth in test lung cancer cell lines, and at 400 μmol/L significantly increased the release of interleukin-6, tumor necrosis factor-alpha, and vascular endothelial growth factor. This agent at 200 and 400 μmol/L also upregulated protein production of ICAM-1, fibronectin, MMP-9, MMP-2, NF-κB p50, p-p38 MAPK, and FAK; and at 400 μmol/L enhanced invasion and migration in test cell lines. These findings suggested that riboflavin at high doses might promote lung cancer progression.

Original languageEnglish
Pages (from-to)H343-H349
JournalJournal of Food Science
Issue number2
Publication statusPublished - Feb 1 2013
Externally publishedYes


  • Cytokines
  • Focal adhesion kinase
  • Migration
  • Non-small cell lung cancer
  • Riboflavin

ASJC Scopus subject areas

  • Food Science


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