Revisiting with a relative-density calibration approach the determination of growth rates of microorganisms by use of optical density data from liquid cultures

Hsiu Li Lin; Chien Chung Lin; Yi Jen Lin; Hsiu-Chen Lin; Chwen-Ming Shih; Chi Rong Chen; Rong Nan Huang; Tai-Chih Kuo

doi:10.1128/AEM.00824-09

Revisiting with a relative-density calibration approach the determination of growth rates of microorganisms by use of optical density data from liquid cultures

Hsiu Li Lin, Chien Chung Lin, Yi Jen Lin, Hsiu-Chen Lin, Chwen-Ming Shih, Chi Rong Chen, Rong Nan Huang, Tai-Chih Kuo

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

To solve the problems of measuring the growth rates of microorganisms from optical density (OD)-growth time plots, we used relative-density (RD) plots. The relationship of OD and RD was built from the diluted grown cultures. This method was satisfactorily applied to study the growth of Escherichia coli and the cyanobacterium Anabaena spiroides.

Original language	English
Pages (from-to)	1683-1685
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	76
Issue number	5
DOIs	https://doi.org/10.1128/AEM.00824-09
Publication status	Published - Mar 2010

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Food Science
Biotechnology
Ecology

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abstract = "To solve the problems of measuring the growth rates of microorganisms from optical density (OD)-growth time plots, we used relative-density (RD) plots. The relationship of OD and RD was built from the diluted grown cultures. This method was satisfactorily applied to study the growth of Escherichia coli and the cyanobacterium Anabaena spiroides.",

author = "Lin, {Hsiu Li} and Lin, {Chien Chung} and Lin, {Yi Jen} and Hsiu-Chen Lin and Chwen-Ming Shih and Chen, {Chi Rong} and Huang, {Rong Nan} and Tai-Chih Kuo",

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AU - Lin, Hsiu Li

AU - Lin, Chien Chung

AU - Lin, Yi Jen

AU - Lin, Hsiu-Chen

AU - Shih, Chwen-Ming

AU - Chen, Chi Rong

AU - Huang, Rong Nan

AU - Kuo, Tai-Chih

PY - 2010/3

Y1 - 2010/3

N2 - To solve the problems of measuring the growth rates of microorganisms from optical density (OD)-growth time plots, we used relative-density (RD) plots. The relationship of OD and RD was built from the diluted grown cultures. This method was satisfactorily applied to study the growth of Escherichia coli and the cyanobacterium Anabaena spiroides.

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Revisiting with a relative-density calibration approach the determination of growth rates of microorganisms by use of optical density data from liquid cultures

Abstract

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