@article{ec5d1e9369a847608cbb9f2acc3a5a13,
title = "Reorganization of cytoskeleton induced by 5-aminolevulinic acid-mediated photodynamic therapy and its correlation with mitochondrial dysfunction",
abstract = "Background and Objectives: This study investigated the early cellular events which occurred after mitochondrial photodamage induced by 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT). Study Design/Materials and Methods: Subcellular localization of protoporphyrin IX (PpIX) in NIH3T3 cells was studied by confocal microscopy. Mitochondrial damage was assessed by measuring mitochondrial transmembrane potential and ATP contents, and confirmed by characteristic appearance on transmission electron microscopy. Cellular adhesion was measured by the level of resistance to trypsinization. Cytoskeletal studies were performed by fluorescent staining of cytoskeletal components. Results: Following ALA-PDT, mitochondrial damage was found in NIH3T3 cells as judged by the decrease of membrane potential and ATP contents. Mitochondrial photodamage was futher confirmed by electron microscopy. Resistance to trypsinization after ALA-PDT was shown to be light dose-dependent. The increase of cellular adhesion after ALA-PDT was correlated with mitochondrial photodamage and reorganization of cytoskeletal components in NIH3T3 cells. Conclusions: This study has demonstrated that mitochondrial dysfunctions induced by ALA-PDT results in alterations of cellular morphology and cellular adhesion. {\textcopyright} 2005 Wiley-Liss, Inc.",
keywords = "Adhesion, Mitochondria, Photodynamic therapy, Protoporphyrin IX, adenosine triphosphate, aminolevulinic acid, fluorescent dye, protoporphyrin, animal cell, article, cell adhesion, cell damage, cell line, confocal microscopy, cytoskeleton, drug effect, membrane potential, mitochondrion, nonhuman, photodynamic therapy, priority journal, transmission electron microscopy, Acid Phosphatase, Adenosine Triphosphate, Aminolevulinic Acid, Animals, Cell Adhesion, Cytoskeleton, Membrane Potentials, Mice, Microscopy, Electron, Transmission, Microscopy, Video, NIH 3T3 Cells, Photochemotherapy, Photosensitizing Agents, Protoporphyrins",
author = "Jui-Chang Tsai and Chia-Lun Wu and Hsiung-Fei Chien and Chin-Tin Chen",
note = "被引用次數：36 Export Date: 16 March 2016 CODEN: LSMED 通訊地址: Chen, C.-T.; Center for Optoelectronic Biomedicine, College of Medicine, National Taiwan University, No. 1, Jen-Ai Road, Section 1st, Taipei 100, Taiwan; 電子郵件： ctchen@ha.mc.ntu.edu.tw 化學物質/CAS: adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; aminolevulinic acid, 106-60-5; protoporphyrin, 553-12-8; Acid Phosphatase, EC 3.1.3.2; Adenosine Triphosphate, 56-65-5; Aminolevulinic Acid, 106-60-5; Photosensitizing Agents; protoporphyrin IX, 553-12-8; Protoporphyrins 參考文獻: Dougherty, T.J., Gomer, C.J., Henderson, B.W., Jori, G., Kessel, D., Korbelik, M., Moan, J., Peng, Q., Photodynamic therapy (1998) J Natl Cancer Inst, 90, pp. 889-905; Dolmans, D.E., Fukumura, D., Jain, R.K., Photodynamic therapy for cancer (2003) Nature Rev Cancer, 3, pp. 380-387; Schuitmaker, J.J., Baas, P., Van Leengoed, H.L., Van Der Meulen, F.W., Star, W.M., Van Zandwijk, N., Photodynamic therapy: A promising new modality for the treatment of cancer (1996) J Photochem Photobiol B, 34, pp. 3-12; Henderson, B.W., Dougherty, T.J., How does photodynamic therapy work? 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year = "2005",
doi = "10.1002/lsm.20179",
language = "English",
volume = "36",
pages = "398--408",
journal = "Lasers in Surgery and Medicine",
issn = "0196-8092",
publisher = "Wiley-Liss Inc.",
number = "5",
}