TY - JOUR
T1 - Reorganization of cytoskeleton induced by 5-aminolevulinic acid-mediated photodynamic therapy and its correlation with mitochondrial dysfunction
AU - Tsai, Jui-Chang
AU - Wu, Chia-Lun
AU - Chien, Hsiung-Fei
AU - Chen, Chin-Tin
N1 - 被引用次數：36
Export Date: 16 March 2016
CODEN: LSMED
通訊地址: Chen, C.-T.; Center for Optoelectronic Biomedicine, College of Medicine, National Taiwan University, No. 1, Jen-Ai Road, Section 1st, Taipei 100, Taiwan; 電子郵件： [email protected]
化學物質/CAS: adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; aminolevulinic acid, 106-60-5; protoporphyrin, 553-12-8; Acid Phosphatase, EC 3.1.3.2; Adenosine Triphosphate, 56-65-5; Aminolevulinic Acid, 106-60-5; Photosensitizing Agents; protoporphyrin IX, 553-12-8; Protoporphyrins
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PY - 2005
Y1 - 2005
N2 - Background and Objectives: This study investigated the early cellular events which occurred after mitochondrial photodamage induced by 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT). Study Design/Materials and Methods: Subcellular localization of protoporphyrin IX (PpIX) in NIH3T3 cells was studied by confocal microscopy. Mitochondrial damage was assessed by measuring mitochondrial transmembrane potential and ATP contents, and confirmed by characteristic appearance on transmission electron microscopy. Cellular adhesion was measured by the level of resistance to trypsinization. Cytoskeletal studies were performed by fluorescent staining of cytoskeletal components. Results: Following ALA-PDT, mitochondrial damage was found in NIH3T3 cells as judged by the decrease of membrane potential and ATP contents. Mitochondrial photodamage was futher confirmed by electron microscopy. Resistance to trypsinization after ALA-PDT was shown to be light dose-dependent. The increase of cellular adhesion after ALA-PDT was correlated with mitochondrial photodamage and reorganization of cytoskeletal components in NIH3T3 cells. Conclusions: This study has demonstrated that mitochondrial dysfunctions induced by ALA-PDT results in alterations of cellular morphology and cellular adhesion. © 2005 Wiley-Liss, Inc.
AB - Background and Objectives: This study investigated the early cellular events which occurred after mitochondrial photodamage induced by 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT). Study Design/Materials and Methods: Subcellular localization of protoporphyrin IX (PpIX) in NIH3T3 cells was studied by confocal microscopy. Mitochondrial damage was assessed by measuring mitochondrial transmembrane potential and ATP contents, and confirmed by characteristic appearance on transmission electron microscopy. Cellular adhesion was measured by the level of resistance to trypsinization. Cytoskeletal studies were performed by fluorescent staining of cytoskeletal components. Results: Following ALA-PDT, mitochondrial damage was found in NIH3T3 cells as judged by the decrease of membrane potential and ATP contents. Mitochondrial photodamage was futher confirmed by electron microscopy. Resistance to trypsinization after ALA-PDT was shown to be light dose-dependent. The increase of cellular adhesion after ALA-PDT was correlated with mitochondrial photodamage and reorganization of cytoskeletal components in NIH3T3 cells. Conclusions: This study has demonstrated that mitochondrial dysfunctions induced by ALA-PDT results in alterations of cellular morphology and cellular adhesion. © 2005 Wiley-Liss, Inc.
KW - Adhesion
KW - Mitochondria
KW - Photodynamic therapy
KW - Protoporphyrin IX
KW - adenosine triphosphate
KW - aminolevulinic acid
KW - fluorescent dye
KW - protoporphyrin
KW - animal cell
KW - article
KW - cell adhesion
KW - cell damage
KW - cell line
KW - confocal microscopy
KW - cytoskeleton
KW - drug effect
KW - membrane potential
KW - mitochondrion
KW - nonhuman
KW - photodynamic therapy
KW - priority journal
KW - transmission electron microscopy
KW - Acid Phosphatase
KW - Adenosine Triphosphate
KW - Aminolevulinic Acid
KW - Animals
KW - Cell Adhesion
KW - Cytoskeleton
KW - Membrane Potentials
KW - Mice
KW - Microscopy, Electron, Transmission
KW - Microscopy, Video
KW - NIH 3T3 Cells
KW - Photochemotherapy
KW - Photosensitizing Agents
KW - Protoporphyrins
U2 - 10.1002/lsm.20179
DO - 10.1002/lsm.20179
M3 - Article
SN - 0196-8092
VL - 36
SP - 398
EP - 408
JO - Lasers in Surgery and Medicine
JF - Lasers in Surgery and Medicine
IS - 5
ER -