Reorganization of cytoskeleton induced by 5-aminolevulinic acid-mediated photodynamic therapy and its correlation with mitochondrial dysfunction

Jui-Chang Tsai, Chia-Lun Wu, Hsiung-Fei Chien, Chin-Tin Chen

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)


Background and Objectives: This study investigated the early cellular events which occurred after mitochondrial photodamage induced by 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT). Study Design/Materials and Methods: Subcellular localization of protoporphyrin IX (PpIX) in NIH3T3 cells was studied by confocal microscopy. Mitochondrial damage was assessed by measuring mitochondrial transmembrane potential and ATP contents, and confirmed by characteristic appearance on transmission electron microscopy. Cellular adhesion was measured by the level of resistance to trypsinization. Cytoskeletal studies were performed by fluorescent staining of cytoskeletal components. Results: Following ALA-PDT, mitochondrial damage was found in NIH3T3 cells as judged by the decrease of membrane potential and ATP contents. Mitochondrial photodamage was futher confirmed by electron microscopy. Resistance to trypsinization after ALA-PDT was shown to be light dose-dependent. The increase of cellular adhesion after ALA-PDT was correlated with mitochondrial photodamage and reorganization of cytoskeletal components in NIH3T3 cells. Conclusions: This study has demonstrated that mitochondrial dysfunctions induced by ALA-PDT results in alterations of cellular morphology and cellular adhesion. © 2005 Wiley-Liss, Inc.
Original languageEnglish
Pages (from-to)398-408
Number of pages11
JournalLasers in Surgery and Medicine
Issue number5
Publication statusPublished - 2005
Externally publishedYes


  • Adhesion
  • Mitochondria
  • Photodynamic therapy
  • Protoporphyrin IX
  • adenosine triphosphate
  • aminolevulinic acid
  • fluorescent dye
  • protoporphyrin
  • animal cell
  • article
  • cell adhesion
  • cell damage
  • cell line
  • confocal microscopy
  • cytoskeleton
  • drug effect
  • membrane potential
  • mitochondrion
  • nonhuman
  • photodynamic therapy
  • priority journal
  • transmission electron microscopy
  • Acid Phosphatase
  • Adenosine Triphosphate
  • Aminolevulinic Acid
  • Animals
  • Cell Adhesion
  • Cytoskeleton
  • Membrane Potentials
  • Mice
  • Microscopy, Electron, Transmission
  • Microscopy, Video
  • NIH 3T3 Cells
  • Photochemotherapy
  • Photosensitizing Agents
  • Protoporphyrins


Dive into the research topics of 'Reorganization of cytoskeleton induced by 5-aminolevulinic acid-mediated photodynamic therapy and its correlation with mitochondrial dysfunction'. Together they form a unique fingerprint.

Cite this