Relevance of A Disintegrin and Metalloproteinase Domain-Containing (ADAM)9 Protein Expression to Bladder Cancer Malignancy

Michika Moriwaki, Trang Thi Huynh Le, Shian Ying Sung, Yura Jotatsu, Youngmin Yang, Yuto Hirata, Aya Ishii, Yi Te Chiang, Kuan Chou Chen, Katsumi Shigemura, Masato Fujisawa

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We evaluated the effect of A Disintegrin and Metalloproteinase Domain-Containing (ADAM)9 protein on exacerbation in bladder cancer KK47 and T24. First, we knocked down ADAM9 and investigated cell proliferation, migration, cell cycle, and the epithelial–mesenchymal transition (EMT)-related proteins expression in vitro. We then investigated the expression level of ADAM9 in clinical urine cytology samples and the Cancer Genome Atlas (TCGA) data. Cell proliferation was significantly reduced in both cell lines after ADAM9 knockdown. In the cell-cycle assay, the percentage of G0/G1 cells was significantly increased in ADAM9 knockdown T24. Migration of T24 was more strongly suppressed than KK47. The expression level of EMT-related proteins suggested that EMT was suppressed in ADAM9 knockdown T24. TCGA analysis revealed that ADAM9 mRNA expression was significantly higher in stage IV and high-grade cancer than in other stages and low-grade cancer. Moreover, in the gene expression omnibus (GEO) study, bladder cancer with surrounding carcinoma and invasive carcinoma showed significantly high ADAM9 mRNA expression. We found that ADAM9 knockdown suppressed cell proliferation and migration in bladder cancer and that high-grade bladder cancer is correlated with higher expression of ADAM9.

Original languageEnglish
Article number791
JournalBiomolecules
Volume12
Issue number6
DOIs
Publication statusPublished - Jun 2022

Keywords

  • A Disintegrin and Metalloproteinase Domain-Containing (ADAM)9 protein
  • bladder cancer
  • epithelial–mesenchymal transition
  • malignancy
  • migration

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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