TY - JOUR
T1 - Regorafenib induces apoptosis and inhibits metastatic potential of human bladder carcinoma cells
AU - Hsu, Fei Ting
AU - Sun, Cho Chin
AU - Wu, Chia Hsing
AU - Lee, Yen Ju
AU - Chiang, Chih Hung
AU - Wang, Wei Shu
PY - 2017/9/1
Y1 - 2017/9/1
N2 - The aim of the present study was to verify the effects of regorafenib on apoptosis and metastatic potential in TSGH 8301 human bladder carcinoma cells in vitro. Cells were treated with different concentration of regorafenib for different periods of time. Effects of regorafenib on cell viability, apoptosis pathways, metastatic potential, and expression of metastatic and anti-apoptotic proteins were evaluated with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay, flow cytometry, cell migration and invasion assay, and western blotting. We found regorafenib significantly reduced cell viability, cell migration and invasion, and expression of metastatic and anti-apoptotic proteins. In addition, regorafenib significantly induced accumulation of sub-G1 phase cells, loss of mitochondrial membrane potential, and expression of active caspase-3 and caspase-8. These results show that regorafenib not only induces apoptosis, but also inhibits metastatic potential in bladder cancer TSGH 8301 cells in vitro.
AB - The aim of the present study was to verify the effects of regorafenib on apoptosis and metastatic potential in TSGH 8301 human bladder carcinoma cells in vitro. Cells were treated with different concentration of regorafenib for different periods of time. Effects of regorafenib on cell viability, apoptosis pathways, metastatic potential, and expression of metastatic and anti-apoptotic proteins were evaluated with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay, flow cytometry, cell migration and invasion assay, and western blotting. We found regorafenib significantly reduced cell viability, cell migration and invasion, and expression of metastatic and anti-apoptotic proteins. In addition, regorafenib significantly induced accumulation of sub-G1 phase cells, loss of mitochondrial membrane potential, and expression of active caspase-3 and caspase-8. These results show that regorafenib not only induces apoptosis, but also inhibits metastatic potential in bladder cancer TSGH 8301 cells in vitro.
KW - Apoptosis
KW - Human bladder carcinoma
KW - Metastatic potential
KW - Regorafenib
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U2 - 10.21873/anticanres.11901
DO - 10.21873/anticanres.11901
M3 - Article
AN - SCOPUS:85029439691
SN - 0250-7005
VL - 37
SP - 4919
EP - 4926
JO - Anticancer Research
JF - Anticancer Research
IS - 9
ER -
