Reduction of Early Growth Response-1 Gene Expression in Osteoblasts by Hydrogen Peroxide

Chia Hua Yang, Ying Li, Wei Jen Chang, Duen Jeng Wang, Sheng Yang Lee, Yoshimitsu Abiko

Research output: Contribution to journalArticlepeer-review


Bone formation steadily declines with age resulting in a loss of bone mass. Reactive oxygen species (ROS) are thought to be major contributors to the aging process. The zinc finger transcription factor, Early Growth Response-1 (Egr-1) is a potential regulator as a transcription factor of many target genes, and plays a role in cell growth, development, and differentiation. However, the effect of ageing on Egr-1 gene expression has not yet been ascertained. To identify which genes have an altered transcription level associated with bone loss by ageing, the pre-osteoblastic cell MC3T3-E1 was treated with H 2O2, and gene expression profiles analyzed with gene chip technology using the Affymetrix GeneChip analysis system (Mouse; 34,000 genes). The expression of many genes in MC3T3-E1 was altered significantly with Egr-1 being decreased by H2O2. The reduction of Egr-1 mRNA levels was successfully confirmed by reversed transcription polymerase chain reaction (RT-PCR) and real-time PCR. Since it has been reported that Egr-1 plays an important role as a transcription factor for growth factor genes which promote cell proliferation and differentiation of preosteoblastic cells, the reduction of Egr-1 gene expression by H2O2 may be involved in the decline of bone formation in the ageing process.

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalJournal of Hard Tissue Biology
Issue number1
Publication statusPublished - 2008


  • Ageing
  • Early growth response-1
  • Osteoblasts

ASJC Scopus subject areas

  • General Dentistry
  • Biochemistry
  • Medicine (miscellaneous)
  • Cell Biology
  • Biomaterials
  • Orthopedics and Sports Medicine


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