Recombinant viral capsid protein VP1 suppresses migration and invasion of human cervical cancer by modulating phosphorylated prohibitin in lipid rafts

Ching Feng Chiu; Jei Ming Peng; Shao Wen Hung; Chi Ming Liang; Shu Mei Liang

doi:10.1016/j.canlet.2012.02.027

Recombinant viral capsid protein VP1 suppresses migration and invasion of human cervical cancer by modulating phosphorylated prohibitin in lipid rafts

Ching Feng Chiu, Jei Ming Peng, Shao Wen Hung, Chi Ming Liang, Shu Mei Liang

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Recombinant capsid protein VP1 (rVP1) of foot-and-mouth disease virus inhibits invasion/metastasis of cancer cells. Here we studied its mechanism of action on human cervical cancer cells. The inhibition of cell invasion by rVP1 was accompanied with reduction in phosphatidylinositol (3,4,5)-triphosphate (PIP3), phospho-Akt S473, phosphorylated prohibitin (phospho-PHB) T258 in lipid rafts, dissociation of phospho-PHB T258 with Raf-1 and the inactivation of Raf-1/ERK. Addition of PIP3 or overexpression of constitutively active Akt and raft-anchored PHB T258 but not PHB T258I mutant protein reversed the inhibitory effects of rVP1. rVP1 inhibited cervical tumor growth and metastasis, and prolonged survival in xenograft mouse models. These results suggest that rVP1 inhibits cancer metastasis via de-phosphorylation of Akt and PHB T258 in lipid rafts to downregulate Raf/ERK signaling.

Original language	English
Pages (from-to)	205-214
Number of pages	10
Journal	Cancer Letters
Volume	320
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2012.02.027
Publication status	Published - Jul 28 2012
Externally published	Yes

Keywords

Cancer metastasis
PIP3
Prohibitin
RVP1
Raf-1

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.canlet.2012.02.027

Cite this

@article{3b81e4ed40df431e98bd313d41931a26,

title = "Recombinant viral capsid protein VP1 suppresses migration and invasion of human cervical cancer by modulating phosphorylated prohibitin in lipid rafts",

abstract = "Recombinant capsid protein VP1 (rVP1) of foot-and-mouth disease virus inhibits invasion/metastasis of cancer cells. Here we studied its mechanism of action on human cervical cancer cells. The inhibition of cell invasion by rVP1 was accompanied with reduction in phosphatidylinositol (3,4,5)-triphosphate (PIP3), phospho-Akt S473, phosphorylated prohibitin (phospho-PHB) T258 in lipid rafts, dissociation of phospho-PHB T258 with Raf-1 and the inactivation of Raf-1/ERK. Addition of PIP3 or overexpression of constitutively active Akt and raft-anchored PHB T258 but not PHB T258I mutant protein reversed the inhibitory effects of rVP1. rVP1 inhibited cervical tumor growth and metastasis, and prolonged survival in xenograft mouse models. These results suggest that rVP1 inhibits cancer metastasis via de-phosphorylation of Akt and PHB T258 in lipid rafts to downregulate Raf/ERK signaling.",

keywords = "Cancer metastasis, PIP3, Prohibitin, RVP1, Raf-1",

author = "Chiu, {Ching Feng} and Peng, {Jei Ming} and Hung, {Shao Wen} and Liang, {Chi Ming} and Liang, {Shu Mei}",

note = "Funding Information: We thank Ms. Chiao-Li Chu for preparing rVP1 and Miranda Loney (Institute of Molecular Biology, Academia Sinica, Taiwan) for English editorial assistance. Grant support: National Science Council , Taiwan (NSC 99-2313-B-001-004-MY3 to S.-M. L) and Academia Sinica (to S.-M. L. and C.-M. L).",

year = "2012",

month = jul,

day = "28",

doi = "10.1016/j.canlet.2012.02.027",

language = "English",

volume = "320",

pages = "205--214",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Recombinant viral capsid protein VP1 suppresses migration and invasion of human cervical cancer by modulating phosphorylated prohibitin in lipid rafts

AU - Chiu, Ching Feng

AU - Peng, Jei Ming

AU - Hung, Shao Wen

AU - Liang, Chi Ming

AU - Liang, Shu Mei

N1 - Funding Information: We thank Ms. Chiao-Li Chu for preparing rVP1 and Miranda Loney (Institute of Molecular Biology, Academia Sinica, Taiwan) for English editorial assistance. Grant support: National Science Council , Taiwan (NSC 99-2313-B-001-004-MY3 to S.-M. L) and Academia Sinica (to S.-M. L. and C.-M. L).

PY - 2012/7/28

Y1 - 2012/7/28

N2 - Recombinant capsid protein VP1 (rVP1) of foot-and-mouth disease virus inhibits invasion/metastasis of cancer cells. Here we studied its mechanism of action on human cervical cancer cells. The inhibition of cell invasion by rVP1 was accompanied with reduction in phosphatidylinositol (3,4,5)-triphosphate (PIP3), phospho-Akt S473, phosphorylated prohibitin (phospho-PHB) T258 in lipid rafts, dissociation of phospho-PHB T258 with Raf-1 and the inactivation of Raf-1/ERK. Addition of PIP3 or overexpression of constitutively active Akt and raft-anchored PHB T258 but not PHB T258I mutant protein reversed the inhibitory effects of rVP1. rVP1 inhibited cervical tumor growth and metastasis, and prolonged survival in xenograft mouse models. These results suggest that rVP1 inhibits cancer metastasis via de-phosphorylation of Akt and PHB T258 in lipid rafts to downregulate Raf/ERK signaling.

AB - Recombinant capsid protein VP1 (rVP1) of foot-and-mouth disease virus inhibits invasion/metastasis of cancer cells. Here we studied its mechanism of action on human cervical cancer cells. The inhibition of cell invasion by rVP1 was accompanied with reduction in phosphatidylinositol (3,4,5)-triphosphate (PIP3), phospho-Akt S473, phosphorylated prohibitin (phospho-PHB) T258 in lipid rafts, dissociation of phospho-PHB T258 with Raf-1 and the inactivation of Raf-1/ERK. Addition of PIP3 or overexpression of constitutively active Akt and raft-anchored PHB T258 but not PHB T258I mutant protein reversed the inhibitory effects of rVP1. rVP1 inhibited cervical tumor growth and metastasis, and prolonged survival in xenograft mouse models. These results suggest that rVP1 inhibits cancer metastasis via de-phosphorylation of Akt and PHB T258 in lipid rafts to downregulate Raf/ERK signaling.

KW - Cancer metastasis

KW - PIP3

KW - Prohibitin

KW - RVP1

KW - Raf-1

UR - http://www.scopus.com/inward/record.url?scp=84859587304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859587304&partnerID=8YFLogxK

U2 - 10.1016/j.canlet.2012.02.027

DO - 10.1016/j.canlet.2012.02.027

M3 - Article

C2 - 22388104

AN - SCOPUS:84859587304

SN - 0304-3835

VL - 320

SP - 205

EP - 214

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Recombinant viral capsid protein VP1 suppresses migration and invasion of human cervical cancer by modulating phosphorylated prohibitin in lipid rafts

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this