Real-time sensing of hepatitis B virus X gene using an ultrasensitive nanowire field effect transistor

Chia Yu Wu, Han Yi Cheng, Keng Liang Ou, Chi Chang Wu

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Devices based on semiconducting nanowires (NWs) are functioning as highly sensitive and selective sensors for the label-free detection of biological and chemical species. This paper demonstrates a novel back-gated silicon NW field effect transistor (NWFET) for gene detection. The fabricated NWFET was employed as the biomolecule sensor for the early, real-time, and label-free screening of hepatitis B virus (HBV) X gene. The DNA fragment in HBV demonstrates the linearity from 10 fM to 1 pM, of which the detection limit is estimated to be about 3.2 fM. The obtained results also show that the NW-based sensor can distinguish the difference between the complementary and 1-base mismatch DNA. The back-gated NW FET exhibits a label-free, highly sensitive, and selective biosensor for gene detection, which also provides a possibility of multiple chemical and biological species detection with sensor array in an integrated chip.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalJournal of Polymer Engineering
Issue number3
Publication statusPublished - May 1 2014


  • field-effect transistor
  • hepatitis B virus
  • label-free
  • nanowire
  • X gene

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics
  • Chemical Engineering(all)


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