Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

Ching Yu Lin; Cheng Chun Kuan; Rong Huay Juang; Samson C.S. Tsou; C. George Kuo; Ching San Chen

Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

Ching Yu Lin, Cheng Chun Kuan, Rong Huay Juang, Samson C.S. Tsou, C. George Kuo, Ching San Chen

Research output: Contribution to journal › Article › peer-review

Abstract

We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.

Original language	English
Pages (from-to)	87-91
Number of pages	5
Journal	Botanical Bulletin of Academia Sinica
Volume	39
Issue number	2
Publication status	Published - 1998
Externally published	Yes

Keywords

Differentially expressed cDNAs
Mungbean
Near isogenic line

ASJC Scopus subject areas

Plant Science

Cite this

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title = "Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean",

abstract = "We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.",

keywords = "Differentially expressed cDNAs, Mungbean, Near isogenic line",

author = "Lin, {Ching Yu} and Kuan, {Cheng Chun} and Juang, {Rong Huay} and Tsou, {Samson C.S.} and Kuo, {C. George} and Chen, {Ching San}",

year = "1998",

language = "English",

volume = "39",

pages = "87--91",

journal = "Botanical Bulletin of Academia Sinica",

issn = "0006-8063",

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T1 - Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

AU - Lin, Ching Yu

AU - Kuan, Cheng Chun

AU - Juang, Rong Huay

AU - Tsou, Samson C.S.

AU - Kuo, C. George

AU - Chen, Ching San

PY - 1998

Y1 - 1998

N2 - We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.

AB - We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.

KW - Differentially expressed cDNAs

KW - Mungbean

KW - Near isogenic line

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M3 - Article

AN - SCOPUS:0031972381

SN - 0006-8063

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EP - 91

JO - Botanical Bulletin of Academia Sinica

JF - Botanical Bulletin of Academia Sinica

IS - 2

ER -

Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

Abstract

Keywords

ASJC Scopus subject areas

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