TY - JOUR
T1 - RAPD analysis of astragalus medicines marketed in Taiwan
AU - Cheng, Kur Ta
AU - Su, Borcherng
AU - Chen, Chien Tsu
AU - Lin, Chun Ching
PY - 2000
Y1 - 2000
N2 - The genetic variability of Astragalus medicine materials sold by twenty randomly selected stores in Taiwan was investigated using RAPD analysis in order to obtain available primers which could clearly differentiate among them. Total DNA isolated from the rhizomes of the samples were used as templates, and sixty 10 mer arbitrary primers were used in the analysis. The aim of the present study is to construct an identification model of molecular biotechniques applicable to Chinese herbal medicines in RAPD analysis. Three of the primers, OPT-03, OPT-13, and OPT-17, revealed polymorphic RAPD fingerprints among the samples of Astragalus membranaceiis, and between Astragalus membranaceits and Hcdysammpolybotrys samples. SSCP analysis was also conducted on PCR products from the ITS-1 region of ribosomal DNA in order to differentiate the two species.
AB - The genetic variability of Astragalus medicine materials sold by twenty randomly selected stores in Taiwan was investigated using RAPD analysis in order to obtain available primers which could clearly differentiate among them. Total DNA isolated from the rhizomes of the samples were used as templates, and sixty 10 mer arbitrary primers were used in the analysis. The aim of the present study is to construct an identification model of molecular biotechniques applicable to Chinese herbal medicines in RAPD analysis. Three of the primers, OPT-03, OPT-13, and OPT-17, revealed polymorphic RAPD fingerprints among the samples of Astragalus membranaceiis, and between Astragalus membranaceits and Hcdysammpolybotrys samples. SSCP analysis was also conducted on PCR products from the ITS-1 region of ribosomal DNA in order to differentiate the two species.
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U2 - 10.1142/S0192415X00000325
DO - 10.1142/S0192415X00000325
M3 - Article
C2 - 10999446
AN - SCOPUS:0033654685
SN - 0192-415X
VL - 28
SP - 273
EP - 278
JO - American Journal of Chinese Medicine
JF - American Journal of Chinese Medicine
IS - 2
ER -