Abstract
Background Previously, we enriched a subpopulation of head and neck cancer-derived tumor initiating cells (HNC-TICs) presented high tumorigenic, chemo-radioresistant, and coupled with epithelial-mesenchymal transition (EMT) properties. The purpose of this study was to investigate the therapeutic effect and molecular mechanisms of quercetin on HNC-TICs. Method ALDH1 activity of head and neck cancer cells with quercetin treatment was assessed by the Aldefluor assay flow cytometry analysis. Self-renewal, invasiveness, and EMT capability of HNC-TICs with different doses of quercetin was presented. Results We first observed that the treatment of quercetin significantly downregulated the ALDH1 activity of head and neck cancer cells in a dose-dependent manner (p <.05). Moreover, quercetin reduced self-renewal property and stemness signatures expression in head and neck cancer-derived sphere cells. The migration ability of head and neck cancer-derived sphere cells was lessened under quercetin treatment partially due to the decreased productions of Twist, N-cadherin, and vimentin. Conclusion Quercetin suppressing HNC-TICs characteristics may therefore be valuable therapeutics clinically in combination with standard treatment modalities.
Original language | English |
---|---|
Pages (from-to) | 413-419 |
Number of pages | 7 |
Journal | Head and Neck |
Volume | 35 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2013 |
Externally published | Yes |
Keywords
- epithelial-mesenchymal transition
- head and neck cancer
- metastasis
- quercetin
- tumor initiating cells
ASJC Scopus subject areas
- Otorhinolaryngology