TY - JOUR
T1 - Quantification and optimization of ethanolic extract containing the bioactive flavonoids from Millettia pulchra radix
AU - Vo, Thanh Hoa
AU - Liaw, Chia Ching
AU - Lin, Yu Chi
AU - Nguyen, Duc Hanh
AU - Nguyen, Thi Tuyet Nhung
AU - Lee, Ching Kuo
AU - Kuo, Yao Haur
N1 - Funding Information:
Funding: This research was funded by the Ministry of Health and Welfare (New Southbound Policy-NRICM-NSP-108-01, NRICM-NSP-109-01), and the Ministry of Science and Technology (MOST104-2320-B-077-006-MY3, MOST107-2320-B-077-003-MY3), Taiwan.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/6/2
Y1 - 2021/6/2
N2 - Millettia pulchra is traditionally used for treating diseases, including joint pain, fever, ane-mia, and allergies. It is also a potential resource of natural flavonoid derivatives, which represents major constituents of this plant. This study aimed to isolate the major compounds from M. pul-chra radix, develop and validate the HPLC-PDA method to determine their contents, and optimize its extraction. Four major flavonoid derivatives (karanjin, lanceolatin B, 2′′,2′′-dimethylpyrano-[5′′,6′′:7,8]-flavone, and pongamol) were isolated using silica gel column chromatography, crystalliza-tion techniques in large amounts with high purities (>95%). A simple, accurate high-performance liquid chromatography–photodiode array (HPLC–PDA) detection method has been developed and validated with significantly statistical impacts according to International Conference on Harmonization (ICH) guidelines. The Response Surface Methodology (RSM), Artificial Neural Network (ANN) models were employed to predictive performance and optimization of the extraction pro-cess. The optimized conditions for the extraction of major flavonoids were: extraction time (twice), solvent/material ratio (9.5), and ethanol concentration (72.5%). Our research suggests an effective method, which will be helpful for quality control in the pharmaceutical development of this species.
AB - Millettia pulchra is traditionally used for treating diseases, including joint pain, fever, ane-mia, and allergies. It is also a potential resource of natural flavonoid derivatives, which represents major constituents of this plant. This study aimed to isolate the major compounds from M. pul-chra radix, develop and validate the HPLC-PDA method to determine their contents, and optimize its extraction. Four major flavonoid derivatives (karanjin, lanceolatin B, 2′′,2′′-dimethylpyrano-[5′′,6′′:7,8]-flavone, and pongamol) were isolated using silica gel column chromatography, crystalliza-tion techniques in large amounts with high purities (>95%). A simple, accurate high-performance liquid chromatography–photodiode array (HPLC–PDA) detection method has been developed and validated with significantly statistical impacts according to International Conference on Harmonization (ICH) guidelines. The Response Surface Methodology (RSM), Artificial Neural Network (ANN) models were employed to predictive performance and optimization of the extraction pro-cess. The optimized conditions for the extraction of major flavonoids were: extraction time (twice), solvent/material ratio (9.5), and ethanol concentration (72.5%). Our research suggests an effective method, which will be helpful for quality control in the pharmaceutical development of this species.
KW - Artificial neural network
KW - Flavonoids
KW - Optimization
KW - Quantification
KW - Response surface methodology
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U2 - 10.3390/molecules26123641
DO - 10.3390/molecules26123641
M3 - Article
C2 - 34203624
AN - SCOPUS:85108841518
SN - 1420-3049
VL - 26
JO - Molecules
JF - Molecules
IS - 12
M1 - 3641
ER -