Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus

Chee Keng Mok; Shih Cheng Chang; Guang Wu Chen; Yu Lun Lo; Shu Jen Chen; Ho Sheng Wu; Ming Tsan Liu; Feng Yee Chang; Tzou Yien Lin; Shin Ru Shih

doi:10.1016/j.jmii.2013.09.010

Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus

Chee Keng Mok, Shih Cheng Chang, Guang Wu Chen, Yu Lun Lo, Shu Jen Chen, Ho Sheng Wu, Ming Tsan Liu, Feng Yee Chang, Tzou Yien Lin, Shin Ru Shih

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.

Original language	English
Pages (from-to)	465-469
Number of pages	5
Journal	Journal of Microbiology, Immunology and Infection
Volume	48
Issue number	4
DOIs	https://doi.org/10.1016/j.jmii.2013.09.010
Publication status	Published - Aug 1 2015
Externally published	Yes

Keywords

Avian influenza A virus
H7N9
Oseltamivir-resistant marker
Pyrosequencing

ASJC Scopus subject areas

Immunology and Allergy
General Immunology and Microbiology
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jmii.2013.09.010

Cite this

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title = "Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus",

abstract = "Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.",

keywords = "Avian influenza A virus, H7N9, Oseltamivir-resistant marker, Pyrosequencing",

author = "Mok, {Chee Keng} and Chang, {Shih Cheng} and Chen, {Guang Wu} and Lo, {Yu Lun} and Chen, {Shu Jen} and Wu, {Ho Sheng} and Liu, {Ming Tsan} and Chang, {Feng Yee} and Lin, {Tzou Yien} and Shih, {Shin Ru}",

note = "Publisher Copyright: {\textcopyright} 2013 Taiwan Society of Microbiology.",

year = "2015",

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doi = "10.1016/j.jmii.2013.09.010",

language = "English",

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AU - Mok, Chee Keng

AU - Chang, Shih Cheng

AU - Chen, Guang Wu

AU - Lo, Yu Lun

AU - Chen, Shu Jen

AU - Wu, Ho Sheng

AU - Liu, Ming Tsan

AU - Chang, Feng Yee

AU - Lin, Tzou Yien

AU - Shih, Shin Ru

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.

AB - Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.

KW - Avian influenza A virus

KW - H7N9

KW - Oseltamivir-resistant marker

KW - Pyrosequencing

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Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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