Purification of growth hormones by reversed-phase high-performance liquid chromatography

Fu Ming Pan; Wen Chang Chang

doi:10.1016/0378-4347(93)80149-X

Purification of growth hormones by reversed-phase high-performance liquid chromatography

Fu Ming Pan
, Wen Chang Chang

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Reversed-phase high-performance liquid chromatography (HPLC) on a column of Radial-Pak C₁₈ cartridge was utilized for the purification of a variety of growth hormone (GH) proteins from mammalian, avian, amphibian and fish pituitary glands. Recovery of GH from pituitary glands of up to 0.43% of total protein was obtained with a high degree of homogeneity as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The HPLC-purified GHs show reactions of identity or near identity by immuno-diffusion studies on agar gel. This method offers a convenient and rapid purification of vertebrate GH on an analytical or preparative scale.

Original language	English
Pages (from-to)	326-329
Number of pages	4
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	613
Issue number	2
DOIs	https://doi.org/10.1016/0378-4347(93)80149-X
Publication status	Published - Apr 2 1993
Externally published	Yes

ASJC Scopus subject areas

General Chemistry

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10.1016/0378-4347(93)80149-X

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abstract = "Reversed-phase high-performance liquid chromatography (HPLC) on a column of Radial-Pak C18 cartridge was utilized for the purification of a variety of growth hormone (GH) proteins from mammalian, avian, amphibian and fish pituitary glands. Recovery of GH from pituitary glands of up to 0.43\% of total protein was obtained with a high degree of homogeneity as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The HPLC-purified GHs show reactions of identity or near identity by immuno-diffusion studies on agar gel. This method offers a convenient and rapid purification of vertebrate GH on an analytical or preparative scale.",

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Purification of growth hormones by reversed-phase high-performance liquid chromatography

Abstract

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