Purification of genomic sized herpesvirus DNA using pulse-field electrophoresis

Robert J. Isfort; Daniel Robinson; Hsing Jien Kung

doi:10.1016/0166-0934(90)90099-2

Purification of genomic sized herpesvirus DNA using pulse-field electrophoresis

Robert J. Isfort, Daniel Robinson, Hsing Jien Kung

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A new method for the separation of genomic sized herpesvirus DNA was developed. This method utilizes the improved separation of extremely high molecular weight DNA molecules by pulse-field electrophoresis. This method has allowed us to separate and purify herpesvirus DNA (> 100 kbp) free of cellular DNA, directly from infected cells. In the case of cell associated herpesviruses (such as Marek's disease herpesvirus), this method is more efficient than present isolation procedures. The DNA obtained is able to be restriction enzyme digested and used in cloning experiments.

Original language	English
Pages (from-to)	311-317
Number of pages	7
Journal	Journal of Virological Methods
Volume	27
Issue number	3
DOIs	https://doi.org/10.1016/0166-0934(90)90099-2
Publication status	Published - Jan 1 1990
Externally published	Yes

Keywords

DNA
Herpesvirus
Pulse-field electrophoresis

ASJC Scopus subject areas

Virology

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10.1016/0166-0934(90)90099-2

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abstract = "A new method for the separation of genomic sized herpesvirus DNA was developed. This method utilizes the improved separation of extremely high molecular weight DNA molecules by pulse-field electrophoresis. This method has allowed us to separate and purify herpesvirus DNA (> 100 kbp) free of cellular DNA, directly from infected cells. In the case of cell associated herpesviruses (such as Marek's disease herpesvirus), this method is more efficient than present isolation procedures. The DNA obtained is able to be restriction enzyme digested and used in cloning experiments.",

keywords = "DNA, Herpesvirus, Pulse-field electrophoresis",

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AU - Robinson, Daniel

AU - Kung, Hsing Jien

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N2 - A new method for the separation of genomic sized herpesvirus DNA was developed. This method utilizes the improved separation of extremely high molecular weight DNA molecules by pulse-field electrophoresis. This method has allowed us to separate and purify herpesvirus DNA (> 100 kbp) free of cellular DNA, directly from infected cells. In the case of cell associated herpesviruses (such as Marek's disease herpesvirus), this method is more efficient than present isolation procedures. The DNA obtained is able to be restriction enzyme digested and used in cloning experiments.

AB - A new method for the separation of genomic sized herpesvirus DNA was developed. This method utilizes the improved separation of extremely high molecular weight DNA molecules by pulse-field electrophoresis. This method has allowed us to separate and purify herpesvirus DNA (> 100 kbp) free of cellular DNA, directly from infected cells. In the case of cell associated herpesviruses (such as Marek's disease herpesvirus), this method is more efficient than present isolation procedures. The DNA obtained is able to be restriction enzyme digested and used in cloning experiments.

KW - DNA

KW - Herpesvirus

KW - Pulse-field electrophoresis

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 3

ER -

Purification of genomic sized herpesvirus DNA using pulse-field electrophoresis

Abstract

Keywords

ASJC Scopus subject areas

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