TY - JOUR
T1 - Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon
AU - Wang, Chung Hsiung
AU - Lo, Chu Fang
AU - Leu, Jiann Horng
AU - Chou, Chih-Ming
AU - Yeh, Pei Yan
AU - Chou, Hsin Yiu
AU - Tung, Min-Chen
AU - Chang, Chen-Fang
AU - Su, Mao-Sen
AU - Kou, Guang Hsiung
PY - 1995
Y1 - 1995
N2 - he causative viral agent was purified from diseased shrimp Penaeus monodon with white spot syndrome. Negatively stained preparations show that the virus is pleiomorphic. It is fusiform or rod-shaped. In negatively stained preparations, the virion measures 70 to 150 nm at its broadest point and is 250 to 380 nm long. In some virions, a tail-like projection extends from one end. The capsid is apparently composed of rings of subunits in a stacked series. The rings are aligned perpendicular to the longitudinal axis of the capsid. The genome of the virus is a double-stranded DNA molecule which produces at least 22 Hind 111 fragments. The full length of the DNA is estimated to be longer than 150 kbp. Based on the morphological characteristics and genomic structures of the virus, we confirm that white spot syndrome associated virus (MJSSV) is a member of genus NOB (Non-Occluded Baculovirus) of the subfamily Nudibaculovirinae of Baculoviridae, name the present isolate PmNOBIII, and propose the use ofWSBV (Baculovirus associated with White Spot syndrome) to indicate PmNOBIII related agents.
AB - he causative viral agent was purified from diseased shrimp Penaeus monodon with white spot syndrome. Negatively stained preparations show that the virus is pleiomorphic. It is fusiform or rod-shaped. In negatively stained preparations, the virion measures 70 to 150 nm at its broadest point and is 250 to 380 nm long. In some virions, a tail-like projection extends from one end. The capsid is apparently composed of rings of subunits in a stacked series. The rings are aligned perpendicular to the longitudinal axis of the capsid. The genome of the virus is a double-stranded DNA molecule which produces at least 22 Hind 111 fragments. The full length of the DNA is estimated to be longer than 150 kbp. Based on the morphological characteristics and genomic structures of the virus, we confirm that white spot syndrome associated virus (MJSSV) is a member of genus NOB (Non-Occluded Baculovirus) of the subfamily Nudibaculovirinae of Baculoviridae, name the present isolate PmNOBIII, and propose the use ofWSBV (Baculovirus associated with White Spot syndrome) to indicate PmNOBIII related agents.
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M3 - Article
SN - 0177-5103
SP - 239
EP - 242
JO - Diseases of Aquatic Organisms
JF - Diseases of Aquatic Organisms
ER -