Purification and characterization of reversible sperm motility inhibitors from porcine seminal plasma

H. Jeng; K. M. Liu; W. C. Chang

doi:10.1006/bbrc.1993.1236

Purification and characterization of reversible sperm motility inhibitors from porcine seminal plasma

H. Jeng, K. M. Liu, W. C. Chang

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Two sperm motility inhibtors (SMI1 and SMI2) were purified from porcine seminal plasma with high performance liquid chromatography (HPLC). Their molecular weights are about 15,000 as estimated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Both of them decreased the percentage of motile spermatozoa in a dose-dependent manner. The inhibitory effect can be abolished by addition of the porcine follicular fluid. Both SMI1 and SMI2 have similar amino acid composition, suggesting that they may be structurally related. They also have inhibitory effect on chymotrypsin.

Original language	English
Pages (from-to)	435-440
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	191
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1993.1236
Publication status	Published - Mar 15 1993
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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Purification and characterization of reversible sperm motility inhibitors from porcine seminal plasma

Abstract

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