TY - JOUR
T1 - Purification and Characterization of Reversible Sperm Motility Inhibitors from Porcine Seminal Plasma
AU - Jeng, H.
AU - Liu, K. M.
AU - Chang, W. C.
PY - 1993/3/15
Y1 - 1993/3/15
N2 - Two sperm motility inhibtors (SMI1 and SMI2) were purified from porcine seminal plasma with high performance liquid chromatography (HPLC). Their molecular weights are about 15,000 as estimated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Both of them decreased the percentage of motile spermatozoa in a dose-dependent manner. The inhibitory effect can be abolished by addition of the porcine follicular fluid. Both SMI1 and SMI2 have similar amino acid composition, suggesting that they may be structurally related. They also have inhibitory effect on chymotrypsin.
AB - Two sperm motility inhibtors (SMI1 and SMI2) were purified from porcine seminal plasma with high performance liquid chromatography (HPLC). Their molecular weights are about 15,000 as estimated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Both of them decreased the percentage of motile spermatozoa in a dose-dependent manner. The inhibitory effect can be abolished by addition of the porcine follicular fluid. Both SMI1 and SMI2 have similar amino acid composition, suggesting that they may be structurally related. They also have inhibitory effect on chymotrypsin.
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U2 - 10.1006/bbrc.1993.1236
DO - 10.1006/bbrc.1993.1236
M3 - Article
C2 - 8461001
AN - SCOPUS:0027205456
SN - 0006-291X
VL - 191
SP - 435
EP - 440
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -