Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines

Chun-Han Chen; Cho Hwa Liao; Ya Ling Chang; Jih Hwa Guh; Shiow Lin Pan; Che Ming Teng

doi:10.1016/j.canlet.2011.09.042

Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines

Chun-Han Chen, Cho Hwa Liao, Ya Ling Chang, Jih Hwa Guh, Shiow Lin Pan, Che Ming Teng

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.

Original language	English
Pages (from-to)	1-11
Number of pages	11
Journal	Cancer Letters
Volume	315
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2011.09.042
Publication status	Published - Feb 1 2012
Externally published	Yes

Keywords

Apoptosis
Cdk1
Microtubules
Mitotic arrest
Protopine

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2011.09.042

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title = "Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines",

abstract = "In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.",

keywords = "Apoptosis, Cdk1, Microtubules, Mitotic arrest, Protopine",

author = "Chun-Han Chen and Liao, {Cho Hwa} and Chang, {Ya Ling} and Guh, {Jih Hwa} and Pan, {Shiow Lin} and Teng, {Che Ming}",

note = "Funding Information: The authors would like to thank Dr. An-Chi Tsai and Miss Chin-Yu Lai for the technical assistance of tube formation assay. This work was supported by research grants of the National Science Council of the Republic of China (NSC 99-2320-B400-008-MY3, NSC 99-2628-B002-024-MY3). Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2012",

month = feb,

day = "1",

doi = "10.1016/j.canlet.2011.09.042",

language = "English",

volume = "315",

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T1 - Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines

AU - Chen, Chun-Han

AU - Liao, Cho Hwa

AU - Chang, Ya Ling

AU - Guh, Jih Hwa

AU - Pan, Shiow Lin

AU - Teng, Che Ming

N1 - Funding Information: The authors would like to thank Dr. An-Chi Tsai and Miss Chin-Yu Lai for the technical assistance of tube formation assay. This work was supported by research grants of the National Science Council of the Republic of China (NSC 99-2320-B400-008-MY3, NSC 99-2628-B002-024-MY3). Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/2/1

Y1 - 2012/2/1

N2 - In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.

AB - In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.

KW - Apoptosis

KW - Cdk1

KW - Microtubules

KW - Mitotic arrest

KW - Protopine

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DO - 10.1016/j.canlet.2011.09.042

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AN - SCOPUS:81955167334

SN - 0304-3835

VL - 315

SP - 1

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JO - Cancer Letters

JF - Cancer Letters

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Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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