Protocol for human placental mesenchymal stem cell therapy in a murine model of intra-abdominal infection of hypervirulent Klebsiella

Li Tzu Wang; Sheng Kang Chiu; Wei Lee; L. Kristopher Siu; Ko Jiunn Liu; Men Luh Yen; B. Linju Yen

doi:10.1016/j.xpro.2021.100337

Protocol for human placental mesenchymal stem cell therapy in a murine model of intra-abdominal infection of hypervirulent Klebsiella

Li Tzu Wang, Sheng Kang Chiu, Wei Lee, L. Kristopher Siu, Ko Jiunn Liu, Men Luh Yen, B. Linju Yen

Research output: Contribution to journal › Article › peer-review

Abstract

Hypervirulent Klebsiella pneumoniae (hvKP) strains cause extra-pulmonary infections such as intra-abdominal infection (IAI) even in healthy individuals due to its resistance to polymorphonuclear neutrophil (PMN) killing and a high incidence of multidrug resistance. To assess whether human placental mesenchymal stem cell (PMSC) therapy can be an effective treatment option, we established a murine model of hvKP-IAI to evaluate immune cell modulation and bacterial clearance for this highly lethal infection. This protocol can rapidly assess potential therapies for severe bacterial IAIs. For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).

Original language	English
Article number	100337
Journal	STAR Protocols
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.100337
Publication status	Published - Mar 19 2021
Externally published	Yes

Keywords

Flow cytometry/mass cytometry
Immunology
Model organisms
Stem cells

ASJC Scopus subject areas

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2021.100337

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title = "Protocol for human placental mesenchymal stem cell therapy in a murine model of intra-abdominal infection of hypervirulent Klebsiella",

abstract = "Hypervirulent Klebsiella pneumoniae (hvKP) strains cause extra-pulmonary infections such as intra-abdominal infection (IAI) even in healthy individuals due to its resistance to polymorphonuclear neutrophil (PMN) killing and a high incidence of multidrug resistance. To assess whether human placental mesenchymal stem cell (PMSC) therapy can be an effective treatment option, we established a murine model of hvKP-IAI to evaluate immune cell modulation and bacterial clearance for this highly lethal infection. This protocol can rapidly assess potential therapies for severe bacterial IAIs. For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).",

keywords = "Flow cytometry/mass cytometry, Immunology, Model organisms, Stem cells",

author = "Wang, {Li Tzu} and Chiu, {Sheng Kang} and Wei Lee and Siu, {L. Kristopher} and Liu, {Ko Jiunn} and Yen, {Men Luh} and Yen, {B. Linju}",

note = "Funding Information: This work was supported in part by funding from NHRI ( 10A1-CSPP06-014 to B.L.Y.), the Ministry of National Defense ROC ( MND-MAB-110-114 to S.K.C.), and the Taiwan Ministry of Science & Technology (MOST: MOST109-2326-B-002 -016 -MY3 to L.T.W., MOST107-2314-B-002-104-MY3 to M.L.Y., and MOST105-2628-B-400-007-MY3 to B.L.Y.). Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

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day = "19",

doi = "10.1016/j.xpro.2021.100337",

language = "English",

volume = "2",

journal = "STAR Protocols",

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T1 - Protocol for human placental mesenchymal stem cell therapy in a murine model of intra-abdominal infection of hypervirulent Klebsiella

AU - Wang, Li Tzu

AU - Chiu, Sheng Kang

AU - Lee, Wei

AU - Siu, L. Kristopher

AU - Liu, Ko Jiunn

AU - Yen, Men Luh

AU - Yen, B. Linju

N1 - Funding Information: This work was supported in part by funding from NHRI ( 10A1-CSPP06-014 to B.L.Y.), the Ministry of National Defense ROC ( MND-MAB-110-114 to S.K.C.), and the Taiwan Ministry of Science & Technology (MOST: MOST109-2326-B-002 -016 -MY3 to L.T.W., MOST107-2314-B-002-104-MY3 to M.L.Y., and MOST105-2628-B-400-007-MY3 to B.L.Y.). Publisher Copyright: © 2021 The Author(s)

PY - 2021/3/19

Y1 - 2021/3/19

N2 - Hypervirulent Klebsiella pneumoniae (hvKP) strains cause extra-pulmonary infections such as intra-abdominal infection (IAI) even in healthy individuals due to its resistance to polymorphonuclear neutrophil (PMN) killing and a high incidence of multidrug resistance. To assess whether human placental mesenchymal stem cell (PMSC) therapy can be an effective treatment option, we established a murine model of hvKP-IAI to evaluate immune cell modulation and bacterial clearance for this highly lethal infection. This protocol can rapidly assess potential therapies for severe bacterial IAIs. For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).

AB - Hypervirulent Klebsiella pneumoniae (hvKP) strains cause extra-pulmonary infections such as intra-abdominal infection (IAI) even in healthy individuals due to its resistance to polymorphonuclear neutrophil (PMN) killing and a high incidence of multidrug resistance. To assess whether human placental mesenchymal stem cell (PMSC) therapy can be an effective treatment option, we established a murine model of hvKP-IAI to evaluate immune cell modulation and bacterial clearance for this highly lethal infection. This protocol can rapidly assess potential therapies for severe bacterial IAIs. For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).

KW - Flow cytometry/mass cytometry

KW - Immunology

KW - Model organisms

KW - Stem cells

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Protocol for human placental mesenchymal stem cell therapy in a murine model of intra-abdominal infection of hypervirulent Klebsiella

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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