Protocol for adeno-associated virus-mediated miRNA delivery in a rat heart failure model

Iqra Mushtaq; Yu Hsun Kao; Yi Jen Chen

doi:10.1016/j.xpro.2024.103498

Protocol for adeno-associated virus-mediated miRNA delivery in a rat heart failure model

Research output: Contribution to journal › Article › peer-review

Abstract

Delivering microRNA (miRNA) to treat cardiac disease is a significant challenge, and selecting an efficient delivery method to target the affected organ is critical for therapeutic success. Here, we present a protocol for delivering miRNA to the heart of rats with heart failure using adeno-associated virus (AAV9) coupled with a hydrodynamic transfusion strategy. We describe steps for inducing heart failure, echocardiography, and AAV9 application. Further, we provide a comprehensive procedure to detect miRNA expression in cardiac tissues using stem-loop RT-qPCR. For complete details on the use and execution of this protocol, please refer to Mushtaq et al.¹

Original language	English
Article number	103498
Journal	STAR Protocols
Volume	5
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2024.103498
Publication status	Published - Dec 20 2024

Keywords

cell biology
gene expression
model organisms
molecular biology
molecular/chemical probes

ASJC Scopus subject areas

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2024.103498

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abstract = "Delivering microRNA (miRNA) to treat cardiac disease is a significant challenge, and selecting an efficient delivery method to target the affected organ is critical for therapeutic success. Here, we present a protocol for delivering miRNA to the heart of rats with heart failure using adeno-associated virus (AAV9) coupled with a hydrodynamic transfusion strategy. We describe steps for inducing heart failure, echocardiography, and AAV9 application. Further, we provide a comprehensive procedure to detect miRNA expression in cardiac tissues using stem-loop RT-qPCR. For complete details on the use and execution of this protocol, please refer to Mushtaq et al.1",

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AB - Delivering microRNA (miRNA) to treat cardiac disease is a significant challenge, and selecting an efficient delivery method to target the affected organ is critical for therapeutic success. Here, we present a protocol for delivering miRNA to the heart of rats with heart failure using adeno-associated virus (AAV9) coupled with a hydrodynamic transfusion strategy. We describe steps for inducing heart failure, echocardiography, and AAV9 application. Further, we provide a comprehensive procedure to detect miRNA expression in cardiac tissues using stem-loop RT-qPCR. For complete details on the use and execution of this protocol, please refer to Mushtaq et al.1

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Protocol for adeno-associated virus-mediated miRNA delivery in a rat heart failure model

Abstract

Keywords

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