TY - JOUR
T1 - Proteomic and functional analysis of zebrafish after administration of antimicrobial peptide epinecidin-1
AU - Huang, Tsui Chin
AU - Chen, Jyh Yih
PY - 2013/2
Y1 - 2013/2
N2 - Antimicrobial peptides (AMPs) play important roles in innate immunity. One such AMP, epinecidin-1, exhibits antibacterial effects in zebrafish. In the current study, we aimed to identify the antimicrobial-associated proteins affected by epinecidin-1 treatment, and to unravel the underlying antimicrobial molecular mechanisms of epinecidin-1. We analyzed proteome changes in epinecidin-1-treated zebrafish using two-dimensional electrophoresis (2DE) coupled to mass spectrometry. Several differentially expressed proteins were identified, some of which were validated by real-time quantitative RT-PCR. The differentially expressed proteins were mapped onto Ingenuity Pathway Analysis canonical pathways, to construct a possible protein-protein interacting network regulated by epinecidin-1; this network suggested a potential role of epinecindin-1 in cytoskeletal assembly and organization. Our findings imply that epinecidin-1 may stabilize the cytoskeleton network in host cells, thereby promoting resistance to bacterial infection.
AB - Antimicrobial peptides (AMPs) play important roles in innate immunity. One such AMP, epinecidin-1, exhibits antibacterial effects in zebrafish. In the current study, we aimed to identify the antimicrobial-associated proteins affected by epinecidin-1 treatment, and to unravel the underlying antimicrobial molecular mechanisms of epinecidin-1. We analyzed proteome changes in epinecidin-1-treated zebrafish using two-dimensional electrophoresis (2DE) coupled to mass spectrometry. Several differentially expressed proteins were identified, some of which were validated by real-time quantitative RT-PCR. The differentially expressed proteins were mapped onto Ingenuity Pathway Analysis canonical pathways, to construct a possible protein-protein interacting network regulated by epinecidin-1; this network suggested a potential role of epinecindin-1 in cytoskeletal assembly and organization. Our findings imply that epinecidin-1 may stabilize the cytoskeleton network in host cells, thereby promoting resistance to bacterial infection.
KW - Antimicrobial peptide
KW - Epinecidin-1
KW - Proteomic
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=84872620529&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872620529&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2012.11.032
DO - 10.1016/j.fsi.2012.11.032
M3 - Article
C2 - 23261508
AN - SCOPUS:84872620529
SN - 1050-4648
VL - 34
SP - 593
EP - 598
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 2
ER -