Protein synthesis-dependent potentiation by thyroxine of antiviral activity of interferon-γ

Hung Yun Lin, Paul M. Yen, Faith B. Davis, Paul J. Davis

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21 Citations (Scopus)


We have studied the prenuclear signal transduction pathway by which thyroid hormone potentiates the antiviral activity of human interferon-γ (IFN-γ) in HeLa cells, which are deficient in thyroid hormone receptor (TR). The action of thyroid hormone was compared with that of milrinone, which has structural homologies with thyroid hormone. L-Thyroxine (T4), 3,5,3'-L- triiodothyronine (T3), and milrinone enhanced the antiviral activity of IFN- γ up to 100-fold; a potentiation blocked by cycloheximide. The 5'- deiodinase inhibitor 6-n-propyl-2-thiouracil did not block the T4 effect. 3,3',5,5'-Tetraiodothyroacetic acid prevented the effect of T4 but not of milrinone. The effects of T4 and milrinone were blocked by inhibitors of protein kinases C (PKC) and A (PKA) and restored by PKC and PKA agonists; only the effect of T was blocked by genistein, a tyrosine kinase inhibitor. In separate models, milrinone was shown not to interact with nuclear TR-β. T4 potentiation of the antiviral activity of IFN-γ requires PKC, PKA, and tyrosine kinase activities but not traditional TR.

Original languageEnglish
Pages (from-to)C1225-C1232
JournalAmerican Journal of Physiology - Cell Physiology
Issue number4 42-4
Publication statusPublished - 1997
Externally publishedYes


  • Protein kinase A
  • Protein kinase C
  • Thyroid hormone action
  • Tyrosine kinase

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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