TY - JOUR
T1 - Protective effect of melatonin on liver ischemia-reperfusion induced pulmonary microvascular injury in rats
AU - Chiu, M. H.
AU - Su, C. L.
AU - Chen, C. F.
AU - Chen, K. H.
AU - Wang, D.
AU - Wang, J. J.
PY - 2012/5
Y1 - 2012/5
N2 - Objective: Reactive oxygen species generated during liver reperfusion have been implicated in remote lung injury. In this study, we evaluate the protective effects of melatonin pretreatment against the increased pulmonary microvascular permeability. Methods: Male Sprague-Dawley rats were divided into three groups: shame-operated, liver ischemia-reperfusion (I/R), and melatonin pretreated (15 mg/kg, intraperitoneally) 15 minutes prior to the liver I/R). The duration of ischemia was 30 minutes, followed by 2 hours of reperfusion. Lungs were isolated in situ and parameters of the capillary filtration coefficient (K fc), lung wet-to-dry weight ratio (W/D), lung weight-to-body weight (LW/BW), and protein concentration in bronchial lavage fluid (PCBAL), the percentage of macrophages and neutrophils in bronchial lavage fluid (BALF), and lung tissue malonedealdehyde were used to assess the lung injury. Results: Liver I/R-induced lung injury was noted by the markedly increased K fc, W/D, LW/BW, PCBAL, and the presence of neutrophils and macrophages in BALF. Lipid peroxidation was also increased (P
AB - Objective: Reactive oxygen species generated during liver reperfusion have been implicated in remote lung injury. In this study, we evaluate the protective effects of melatonin pretreatment against the increased pulmonary microvascular permeability. Methods: Male Sprague-Dawley rats were divided into three groups: shame-operated, liver ischemia-reperfusion (I/R), and melatonin pretreated (15 mg/kg, intraperitoneally) 15 minutes prior to the liver I/R). The duration of ischemia was 30 minutes, followed by 2 hours of reperfusion. Lungs were isolated in situ and parameters of the capillary filtration coefficient (K fc), lung wet-to-dry weight ratio (W/D), lung weight-to-body weight (LW/BW), and protein concentration in bronchial lavage fluid (PCBAL), the percentage of macrophages and neutrophils in bronchial lavage fluid (BALF), and lung tissue malonedealdehyde were used to assess the lung injury. Results: Liver I/R-induced lung injury was noted by the markedly increased K fc, W/D, LW/BW, PCBAL, and the presence of neutrophils and macrophages in BALF. Lipid peroxidation was also increased (P
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U2 - 10.1016/j.transproceed.2012.01.097
DO - 10.1016/j.transproceed.2012.01.097
M3 - Article
C2 - 22564597
AN - SCOPUS:84860726956
SN - 0041-1345
VL - 44
SP - 962
EP - 965
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 4
ER -