TY - JOUR
T1 - Protective effect of a gonadotropin-releasing hormone analogue on chemotherapeutic agent-induced ovarian gonadotoxicity
T2 - A mouse model
AU - Tan, Shun Jen
AU - Yeh, Yu Chi
AU - Shang, Wei Jen
AU - Wu, Gwo Jang
AU - Liu, Jah Yao
AU - Chen, Chi Huang
PY - 2010/4
Y1 - 2010/4
N2 - Objective: To demonstrate the protective effect of triptorelin, a GnRH analogue, on chemotherapy-induced ovarian gonadotoxicity. Study design: Twenty-four sexually mature, virgin, female FVB/NJNarl mice were divided into four groups: busulfan (B); low-dose triptorelin plus busulfan (T L + B); high-dose triptorelin plus busulfan (T H + B); and control. Mice in the T L + B and T H + B groups were injected with 3.8 and 38 mg/kg of triptorelin subcutaneously, respectively. Four weeks later, mice in the B, T L + B, and T H + B groups were injected with busulfan intraperitoneally at a dose of 36 mg/kg. Histologic examinations were performed 4 weeks later. Results: Obvious destruction of ovarian structure and significant depletion of primordial, primary, and secondary follicles were demonstrated in the B group compared with the control group, affirming the gonadotoxicity of busulfan. In the T L + B group, a greater number of larger primordial and primary follicles were enumerated compared with the B group; however, statistical significance was not achieved. In the T H + B group, the number of primordial and primary follicles was significantly greater than in the B group, and the ovarian tissue in the T H + B group was spared, demonstrating the effect of triptorelin pre-treatment on ovarian protection. Conclusion: Our results have demonstrated a dose-dependent protective effect against gonadotoxic chemotherapy of a GnRH analogue on ovarian reserve, thus suggesting a novel application of GnRH analogues in fertility preservation. Crown
AB - Objective: To demonstrate the protective effect of triptorelin, a GnRH analogue, on chemotherapy-induced ovarian gonadotoxicity. Study design: Twenty-four sexually mature, virgin, female FVB/NJNarl mice were divided into four groups: busulfan (B); low-dose triptorelin plus busulfan (T L + B); high-dose triptorelin plus busulfan (T H + B); and control. Mice in the T L + B and T H + B groups were injected with 3.8 and 38 mg/kg of triptorelin subcutaneously, respectively. Four weeks later, mice in the B, T L + B, and T H + B groups were injected with busulfan intraperitoneally at a dose of 36 mg/kg. Histologic examinations were performed 4 weeks later. Results: Obvious destruction of ovarian structure and significant depletion of primordial, primary, and secondary follicles were demonstrated in the B group compared with the control group, affirming the gonadotoxicity of busulfan. In the T L + B group, a greater number of larger primordial and primary follicles were enumerated compared with the B group; however, statistical significance was not achieved. In the T H + B group, the number of primordial and primary follicles was significantly greater than in the B group, and the ovarian tissue in the T H + B group was spared, demonstrating the effect of triptorelin pre-treatment on ovarian protection. Conclusion: Our results have demonstrated a dose-dependent protective effect against gonadotoxic chemotherapy of a GnRH analogue on ovarian reserve, thus suggesting a novel application of GnRH analogues in fertility preservation. Crown
KW - Fertility preservation
KW - GnRH analogue
KW - Gonadotoxicity
KW - Ovarian protection
KW - Primordial follicles
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U2 - 10.1016/j.ejogrb.2009.12.028
DO - 10.1016/j.ejogrb.2009.12.028
M3 - Article
C2 - 20074846
AN - SCOPUS:77549087226
SN - 0301-2115
VL - 149
SP - 182
EP - 185
JO - European Journal of Obstetrics and Gynecology and Reproductive Biology
JF - European Journal of Obstetrics and Gynecology and Reproductive Biology
IS - 2
ER -