TY - JOUR
T1 - Protection in rats with heatstroke
T2 - Hyperbaric oxygen vs activated protein C therapy
AU - Yeh, Chao Hung
AU - Chen, Zhih Cherng
AU - Hsu, Chuan Chih
AU - Lin, Mao Tsun
AU - Chen, Chien Chang
N1 - Funding Information:
This work was supported by the National Science Council of the Republic of China (Taiwan) (Grant nos. NSC96-2320-B-218-MY2 ; NSC96-2314-B-384-003-MY3 ; NSC96-2314-B-384-006-MY3 ).
PY - 2010/6
Y1 - 2010/6
N2 - The present study was attempted to evaluate the therapeutic effects of activated protein C and/or hyperbaric oxygen in an animal model of heatstroke. Sixty-eight minutes heat stress (43°C) initiated, the anesthetized rats were randomized to several groups and administered: 1) no resuscitation (vehicle solution plus normabaric air, 2) intravenous activated protein C (1. mg in 1. ml of normal saline per kg of body weight), 3) hyperbaric oxygen (100% oxygen at 202. kpa for 17. min), and 4) intravenous activated protein C plus hyperbaric oxygen. Another group of rats exposed to room temperature (26°C) was used as normothermic controls. Blood sampling was 0. min, 70. min, and 85. min after heat stress initiated. When the vehicle-treated rats underwent heat exposure, their survival time values found were to be 19-25. min. Resuscitation with activated protein C or hyperbaric oxygen significantly and equally improved survival during heatstroke (134-159. min). As compared with those of activated protein C or hyperbaric oxygen alone, combined activated protein C and hyperbaric oxygen significantly had higher survival time values (277-347. min). All vehicle-treated heatstroke animals displayed systemic response, hypercoagulable state, and hepatic and renal dysfunction. Combined activated protein C and hyperbaric oxygen therapy reduced these heatstroke reactions better than activated protein C or hyperbaric oxygen alone. The results indicate consequently, combined activated protein C and hyperbaric oxygen therapy heightens benefit in combating heatstroke reactions.
AB - The present study was attempted to evaluate the therapeutic effects of activated protein C and/or hyperbaric oxygen in an animal model of heatstroke. Sixty-eight minutes heat stress (43°C) initiated, the anesthetized rats were randomized to several groups and administered: 1) no resuscitation (vehicle solution plus normabaric air, 2) intravenous activated protein C (1. mg in 1. ml of normal saline per kg of body weight), 3) hyperbaric oxygen (100% oxygen at 202. kpa for 17. min), and 4) intravenous activated protein C plus hyperbaric oxygen. Another group of rats exposed to room temperature (26°C) was used as normothermic controls. Blood sampling was 0. min, 70. min, and 85. min after heat stress initiated. When the vehicle-treated rats underwent heat exposure, their survival time values found were to be 19-25. min. Resuscitation with activated protein C or hyperbaric oxygen significantly and equally improved survival during heatstroke (134-159. min). As compared with those of activated protein C or hyperbaric oxygen alone, combined activated protein C and hyperbaric oxygen significantly had higher survival time values (277-347. min). All vehicle-treated heatstroke animals displayed systemic response, hypercoagulable state, and hepatic and renal dysfunction. Combined activated protein C and hyperbaric oxygen therapy reduced these heatstroke reactions better than activated protein C or hyperbaric oxygen alone. The results indicate consequently, combined activated protein C and hyperbaric oxygen therapy heightens benefit in combating heatstroke reactions.
KW - Coagulation
KW - Heatstroke
KW - Hyperbaric oxygen
KW - Inflammation
KW - Multiorgan dysfunction
KW - Protein C
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U2 - 10.1016/j.ejphar.2010.01.013
DO - 10.1016/j.ejphar.2010.01.013
M3 - Article
C2 - 20123096
AN - SCOPUS:77952319369
SN - 0014-2999
VL - 635
SP - 103
EP - 108
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1-3
ER -