Propofol reduces nitric oxide biosynthesis in lipopolysaccharide-activated macrophages by downregulating the expression of inducible nitric oxide synthase

Ruei-Ming Chen; Gong-Jhe Wu; Yu-Ting Tai; Wei Zen Sun; Yi Ling Lin; Wen Chi Jean; Ta-Liang Chen

doi:10.1007/s00204-003-0453-z

Propofol reduces nitric oxide biosynthesis in lipopolysaccharide-activated macrophages by downregulating the expression of inducible nitric oxide synthase

Ruei-Ming Chen, Gong-Jhe Wu, Yu-Ting Tai, Wei Zen Sun, Yi Ling Lin, Wen Chi Jean, Ta-Liang Chen

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

Nitric oxide is an active oxidant that contributes to the physiology and pathophysiology of macrophages. Propofol has been widely used in intravenous anesthesia. It possess antioxidant and immunomodulating effects. This study aimed to evaluate the effects of propofol on nitric oxide production in lipopolysaccharide-activated macrophages. Exposure of macrophages to propofol (25, 50 and 75 μM), to lipopolysaccharide (0.5, 1, 1.5 and 2 ng/ml) or to a combination ofpropofol and lipopolysaccharide did not affect cell viability. However, propofol at 100 μM led to significant cell death (P3 versus 92±6×10³ arbitrary units, P2 versus 30±4×10² arbitrary units, P

Original language	English
Pages (from-to)	418-423
Number of pages	6
Journal	Archives of Toxicology
Volume	77
Issue number	7
DOIs	https://doi.org/10.1007/s00204-003-0453-z
Publication status	Published - Jul 1 2003

Keywords

Inducible nitric oxide synthase
Lipopoly saccharide
Macrophages
Nitric oxide
Propofol

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

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title = "Propofol reduces nitric oxide biosynthesis in lipopolysaccharide-activated macrophages by downregulating the expression of inducible nitric oxide synthase",

abstract = "Nitric oxide is an active oxidant that contributes to the physiology and pathophysiology of macrophages. Propofol has been widely used in intravenous anesthesia. It possess antioxidant and immunomodulating effects. This study aimed to evaluate the effects of propofol on nitric oxide production in lipopolysaccharide-activated macrophages. Exposure of macrophages to propofol (25, 50 and 75 μM), to lipopolysaccharide (0.5, 1, 1.5 and 2 ng/ml) or to a combination ofpropofol and lipopolysaccharide did not affect cell viability. However, propofol at 100 μM led to significant cell death (P3 versus 92±6×103 arbitrary units, P2 versus 30±4×102 arbitrary units, P",

keywords = "Inducible nitric oxide synthase, Lipopoly saccharide, Macrophages, Nitric oxide, Propofol",

author = "Ruei-Ming Chen and Gong-Jhe Wu and Yu-Ting Tai and Sun, {Wei Zen} and Lin, {Yi Ling} and Jean, {Wen Chi} and Ta-Liang Chen",

note = "Funding Information: Acknowledgements The authors express their gratitude to Ms Sheau-Lan Tzeng and Ms Wan-Ju Lee for their technical support and data collection of the experiment. This study is supported by grants TMU90-Y05-A123 from Taipei Medical University and NSC90-2314-B-038-045 from the National Science Council, Taiwan, ROC.",

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doi = "10.1007/s00204-003-0453-z",

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AU - Chen, Ruei-Ming

AU - Wu, Gong-Jhe

AU - Tai, Yu-Ting

AU - Sun, Wei Zen

AU - Lin, Yi Ling

AU - Jean, Wen Chi

AU - Chen, Ta-Liang

N1 - Funding Information: Acknowledgements The authors express their gratitude to Ms Sheau-Lan Tzeng and Ms Wan-Ju Lee for their technical support and data collection of the experiment. This study is supported by grants TMU90-Y05-A123 from Taipei Medical University and NSC90-2314-B-038-045 from the National Science Council, Taiwan, ROC.

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Nitric oxide is an active oxidant that contributes to the physiology and pathophysiology of macrophages. Propofol has been widely used in intravenous anesthesia. It possess antioxidant and immunomodulating effects. This study aimed to evaluate the effects of propofol on nitric oxide production in lipopolysaccharide-activated macrophages. Exposure of macrophages to propofol (25, 50 and 75 μM), to lipopolysaccharide (0.5, 1, 1.5 and 2 ng/ml) or to a combination ofpropofol and lipopolysaccharide did not affect cell viability. However, propofol at 100 μM led to significant cell death (P3 versus 92±6×103 arbitrary units, P2 versus 30±4×102 arbitrary units, P

AB - Nitric oxide is an active oxidant that contributes to the physiology and pathophysiology of macrophages. Propofol has been widely used in intravenous anesthesia. It possess antioxidant and immunomodulating effects. This study aimed to evaluate the effects of propofol on nitric oxide production in lipopolysaccharide-activated macrophages. Exposure of macrophages to propofol (25, 50 and 75 μM), to lipopolysaccharide (0.5, 1, 1.5 and 2 ng/ml) or to a combination ofpropofol and lipopolysaccharide did not affect cell viability. However, propofol at 100 μM led to significant cell death (P3 versus 92±6×103 arbitrary units, P2 versus 30±4×102 arbitrary units, P

KW - Inducible nitric oxide synthase

KW - Lipopoly saccharide

KW - Macrophages

KW - Nitric oxide

KW - Propofol

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Propofol reduces nitric oxide biosynthesis in lipopolysaccharide-activated macrophages by downregulating the expression of inducible nitric oxide synthase

Abstract

Keywords

ASJC Scopus subject areas

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